Mitochondrial cysteinyl-tRNA synthetase is expressed via alternative transcriptional initiation regulated by energy metabolism in yeast cells

Eukaryotes typically utilize two distinct aminoacyl-tRNA synthetase isoforms, one for cytosolic and one for mitochondrial protein synthesis. However, the genome of budding yeast (Saccharomyces cerevisiae) contains only one cysteinyl-tRNA synthetase gene (YNL247W, also known as CRS1). In this study, we report that CRS1 encodes both cytosolic and mitochondrial isoforms. The 5′ complementary DNA end method and GFP reporter gene analyses indicated that yeast CRS1 expression yields two classes of mRNAs through alternative transcription starts: a long mRNA containing a mitochondrial targeting sequence and a short mRNA lacking this targeting sequence. We found that the mitochondrial Crs1 is the product of translation from the first initiation AUG codon on the long mRNA, whereas the cytosolic Crs1 is produced from the second in-frame AUG codon on the short mRNA. Genetic analysis and a ChIP assay revealed that the transcription factor heme activator protein (Hap) complex, which is involved in mitochondrial biogenesis, determines the transcription start sites of the CRS1 gene. We also noted that Hap complex–dependent initiation is regulated according to the needs of mitochondrial energy production. The results of our study indicate energy-dependent initiation of alternative transcription of CRS1 that results in production of two Crs1 isoforms, a finding that suggests Crs1's potential involvement in mitochondrial energy metabolism in yeast.

Aminoacyl-tRNA synthetase (ARS) 2 is an ancestral housekeeping enzyme found in all organisms. ARSs are essential for precise translation of the genetic code and required for correct ligation of an amino acid to its cognate tRNA, thereby forming aminoacyl-tRNA (1). These aminoacyl-tRNAs are used as substrates for codon recognition and polymerization of amino acids in ribosomes. Prokaryotes typically have 20 ARSs that are responsible for amino acid aminoacylation and production of aminoacyl-tRNAs. In eukaryotes, the translation events occur in both the cytosol and mitochondria (2,3). Hence, eukaryotes commonly use two distinct sets of ARSs: one functioning in the cytosol and the other operating in mitochondria. Mitochondrial ARSs are encoded by nuclear DNA, are translated in the cytosol, and are transported to organelles (mitochondria), unlike cytosolic ARSs. Mitochondrial ARSs are generally encoded by distinct genes different from those encoding cytosolic ARSs (4,5). Some ARSs are encoded by single genes. All ARS genes can typically be distinguished on the basis of phylogenetic analyses, which shows that most mitochondrial ARSs derive from proteobacterial ARSs, but, in some cases, the proteobacterial endosymbiotic origins of the mitochondrial ARSs are unknown, possibly because of gene replacements, intranuclear gene duplications, and horizontal gene transfers (6). The exact alternative mechanisms of substitution for such missing ARS genes have remained unclear.
Some ARSs reportedly function in physiological processes other than translation, called moonlighting functions (7,8). For example, although Cys-tRNA synthetase (CysRS, also called CARS) mediates the translation process by catalyzing Cys-tRNA synthesis (i.e. a canonical function), our earlier study showed that CysRS has another important function: to catalyze the biosynthesis of cysteine persulfides; i.e. it acts as a cysteine persulfide synthase (CPERS) (9). Therefore, the CPERS activity of CysRS is a new function of ARSs beyond translation, which is, in fact, the major pathway for production of endogenous reactive persulfides that is observed in many organisms. In the context of this unique persulfide function, we recently found that cysteine persulfides produced by CysRS in mitochondria can support and up-regulate mitochondrial biogenesis and bioenergetics in eukaryotes (9,10). Therefore, mitochondrial transport and localization of CysRS are presumably crucial steps for energy metabolism in addition to translation in mitochondria, although this view is still hypothetical and needs to be verified.
Nuclearly encoded mitochondrial proteins commonly contain targeting signals at their N termini, called mitochondrial targeting signals (MTSs), which localize them in mitochondria; these MTSs constitute proteolytically cleavable sequences or N termini of mature proteins that remain after being transported and located in mitochondria (11,12). MTSs manifest great variety in their lengths and sequences (13). In fact, no sequence conservation or structural motifs of MTSs have been identified for mitochondrial proteins. The only common feature of MTSs is that they contain basic, hydroxylated, and hydrophobic residues that confer a tendency to form an ␣-helices (13). This amphipathic nature is thought to play an important role in the recognition of mitochondrial receptors for transportation.
The genome of Saccharomyces cerevisiae lacks specific mitochondrial ARSs catalyzing aminoacylation of Ala, Gln, His, Val, and Cys for tRNA. In these cases, both cytosolic and mitochondrial isoforms are encoded and translated by a single gene via different mechanisms: alternative transcription initiation, as shown for the His-tRNA synthetase (14,16) and Val-tRNA synthetase (15), and alternative translation started at a noncanonical initiation codon, as documented for the Ala-tRNA synthetase (17). These alternative pathways for aminoacyl-tRNA synthesis allow expression of MTS-containing ARSs (mitochondrial types) and ARSs without MTS (cytosolic types) from single genes of individual ARSs. However, the regulatory mechanism for the alternative yeast CysRS protein biosynthesis remains unclear.
In this study, we investigated the mitochondrial localization mechanism of CysRS (gene YNL247W, called CRS1 here). Our study indicates that, although CRS1 encodes both cytosolic and mitochondrial isoforms, transcription and translation of the mitochondrial CysRS are activated by an alternative transcription initiation of the single CRS1 gene. This translational activation of CRS1 is governed by a key transcription factor, i.e. a heme activator protein (Hap) complex that critically regulates the expression of many genes encoding proteins involved in energy metabolism and biogenesis in yeast mitochondria. Our data therefore demonstrate mitochondrial expression of Crs1 in this yeast that is specifically controlled by the Hap complex, a master regulator of mitochondrial bioenergetics and biogenesis in this eukaryotic organism in vivo.

Results
The genome of the yeast has only one CysRS gene (CRS1), which suggests that CRS1 may encode both cytosolic and mitochondrial isoforms. To investigate the subcellular localization of CysRS, we first prepared a mutant yeast strain by inserting a GFP cDNA into the 3Ј site of CRS1 in the genome of the yeast (hereafter called WT-GFP), which resulted in production of the fusion protein Crs1-GFP. This mutant yeast was used for livecell imaging, which revealed a clear GFP signal for Crs1-GFP in both the cytosol and mitochondria (Fig. 1A). Western blot anal-ysis confirmed expression of Crs1-GFP in both cytosolic and mitochondrial fractions (Fig. 1B). Earlier studies showed that a single gene encoded both cytosolic and mitochondrial isoforms, which are expressed by one of two different mechanisms: alternative transcription or translation starting at the noncanonical start codon (ACG) (14 -17). However, the CRS1 sequence in this yeast contained no in-frame noncanonical start codon. Hence, we sought to determine the possible involvement of an alternative transcription for the CRS1 gene in mitochondrial Crs1 expression. To determine the transcription start sites of CRS1, we used the rapid amplification of 5Ј cDNA ends method (5Ј-RACE) with mRNA extracted from WT cells. 5Ј-RACE indicated that CRS1 mRNAs were largely divided into two types, long and short mRNAs, in terms of the translation initiation codon (AUG) (Fig. 1C). The long mRNA contained an additional 39 bp (ATGAATATCTTCATAAAA-GCCCTGAGAAGATATACTATA) at the 5Ј position compared with the short mRNA. The N-terminal peptide (MNI-FIKALRRYTI) translated from this additional sequence had basic and hydrophobic residues. A protein secondary structure prediction software (JPred 4 http://www.compbio.dundee. ac.uk/jpred4) 3 (18) indicated that the peptide formed amphipathic ␣-helices, which are common features of MTSs; that is, the long Crs1 isoform thereby translated was probably transported into mitochondria, whereas the short Crs1 isoform may be located in the cytosol. For analysis of the functions of the additional N-terminal peptide, a plasmid containing the long CRS1 and one containing the short CRS1, each with the alcohol dehydrogenase 1 (ADH1) promoter, were constructed and expressed in WT cells (Fig. 1D). Laser confocal microscopy analysis showed that Crs1 (Crs1full) that was translated from the long CRS1 was located only in mitochondria. The N-terminal truncated Crs1 (Crs1⌬N13) that was translated from the short CRS1, however, remained only in the cytosol. Western blot analysis also indicated that Crs1full was located only in mitochondria, but Crs1⌬N13 remained only in the cytosol, confirming the results of the fluorescence microscopy analysis (Fig. 1E).
To perform additional analyses of the N-terminal sequence, we produced a Crs1 N-terminal truncated strain (⌬N13-GFP) from WT-GFP by using the CRISPR-Cas9 system (Fig. S1A). Confocal microscopy analysis showed that Crs1 in WT-GFP was found in both the cytosol and mitochondria but that the N-terminal truncated Crs1 signal in ⌬N13-GFP occurred only in the cytosol ( Fig. 2A). Western blot analysis confirmed that the N-terminal truncated Crs1 in ⌬N13-GFP was located only in the cytosol (Fig. 2B). Next we investigated the translation activity in mitochondria of ⌬N13-GFP by analyzing the expression of mitochondrial genome-encoded genes. Western blotting for ⌬N13-GFP indicated that the expression of mitochondrial porin (Por1), a nuclearly encoded gene, was intact, whereas the expression of subunit II of cytochrome c oxidase (Cox2), a mitochondrially encoded gene, was completely nullified (Fig. 2C). Moreover, we tested the growth of ⌬N13-GFP in glucose or glycerol as a carbon source. When yeast is cultured in fermentable carbon sources (glucose), energy production occurs mainly via glycolysis, independent of mitochondrial respiration (19). In contrast, energy production for growth in nonfermentable carbon sources (glycerol) is well-known to depend completely on mitochondrial oxidative phosphorylation. ⌬N13-GFP cells grew normally in glucose medium but showed defective growth in glycerol medium, which indicated that mitochondrial oxidative phosphorylation was impaired and insufficient to maintain cellular bioenergetics because of the loss of translation activity in mitochondria (Fig. 2D). These results suggest that CRS1 encodes both cytosolic and mitochondrial isoforms via alternative transcription from a single gene and that the N-terminal peptide in the protein translated from the long mRNA probably functions as an MTS.
When yeast cells are grown in medium containing glucose, they metabolize glucose predominantly by glycolysis, which releases ethanol into the medium. When the glucose supply becomes limited, cells undergo a diauxic shift characterized by a reduced growth rate and by switching metabolism from glycolysis to aerobic utilization of ethanol via the tricarboxylic acid cycle and oxidative phosphorylation in the mitochondria (20 -22). Basically, although yeast growth is independent of mitochondria before the diauxic shift, it depends fully on mitochondria after the diauxic shift (Fig. S2). We used real-time quantitative PCR (qPCR) to develop a system to detect the long mRNA (mitochondrial mRNA) of CRS1 (Fig. S3). This qPCR analysis showed that the mitochondrial mRNA level increased as yeast cells grew on glucose medium (Fig. 3A). In fact, the mitochondrial CRS1 mRNA level increased rapidly from early stationary phase after the diauxic shift was initiated. We also found that mitochondrial mRNA expression increased even The cells were grown in YPGly to log phase, and then the purified mitochondrial fractions were isolated. Protein samples (5 g) were prepared for detection of Crs1-GFP, Pgk1, and Por1. C, transcription start site analysis of CRS1. Multiple transcription start sites were determined via 5Ј-RACE analysis (n ϭ 150). The x axis indicates the location of each mRNA start site and the y axis the number of times the sequence was found. The schematic at the top represents part of the CRS1 mRNA. The uppercase letters indicate the nucleotides of the transcription start sites, and the boxes indicate the nucleotides comprising the start codons. The translation start site in the database (RefSeq ID NM_001183085.1) is indicated by ϩ1. D, localization analysis of Crs1 expressed with a nonoriginal promoter. WT cells harboring the pCRS1full-GFP or pCRS1⌬N13-GFP were found using fluorescence microscopy. pCRS1full-GFP is a plasmid that expresses Crs1 tagged with GFP at the C terminus plus the ADH1 promoter. pCRS1⌬N13-GFP is a plasmid that expresses the N-terminal truncated Crs1 tagged with GFP plus the ADH1 promoter. The schematic on the right represents the plasmid structures of pCRS1full-GFP and pCRS1⌬N13-GFP used here. MitoTracker Red was used to stain mitochondria. E, Western blot analysis of Crs1 expressed with a nonoriginal promoter. WT cells harboring pCRS1full-GFP or pCRS1⌬N13-GFP were grown in SG without uracil to log phase, and the whole, cytosolic, and mitochondrial fractions were isolated. Protein samples (10 g) were prepared for detection of Crs1-GFP, Pgk1, and Por1.

Mitochondrial yeast cysteinyl-tRNA synthetase and bioenergy
during the log phase of growth in culture medium containing glycerol as a nonfermentable carbon source (Fig. 3A). These results thus indicated that regulation of mitochondrial mRNA transcription depended on the energy metabolism demand of the cells. Several reports proposed that mitochondrial bioenergetics and biogenesis were regulated by some transcription factors, especially Hap2, Rtg1, and Mig2 (23, 24). We thus investigated whether these factors were involved in the transcription of mitochondrial CRS1. As Fig. 3B shows, deletion of HAP2 caused loss of the increased mitochondrial CRS1 mRNA depending on the growth phase, whereas ⌬rtg1 and ⌬mig2 showed results similar to that of the WT. In addition, the absence of any subunits of the Hap complex led to a defect in the increase of mitochondrial CRS1 mRNA expression (Fig.  3C). We searched the Hap complex-binding site (CCAAT) upstream of the CRS1 gene (chromosome XIV 182074_182078) via sequence analysis (25) and constructed a strain (⌬hap-BS) that is deficient in the Hap complex-binding site in the genome by using the CRISPR-Cas9 system (Fig. S1B). ⌬hap-BS thus obtained was defective in the increase in mitochondrial CRS1 mRNA, as were the disrupted mutants of the HAP gene (Fig.  3C). Next we observed the effect of overexpression of HAP4, the gene for the catalytic subunit of the Hap complex, on the transcription of mitochondrial CRS1 mRNA. Fig. 3D shows that mitochondrial CRS1 mRNA in HAP4-overexpressing cells was transcribed even during the log phase of growth in culture medium containing glucose. We then used a ChIP assay with Hap2 for Hap complex-binding sites upstream of the CRS1 gene. Fig. 3E shows that Hap2 bound to the Hap complexbinding site only during late stationary phase in glucose medium. In contrast, during growth in glycerol medium, Hap2 bound to the Hap complex-binding site during any phase, even log phase. These results therefore indicate that the Hap complex may regulate the transcription start site of CRS1 mRNA by directly binding to the promoter of the CRS1 gene.
A transcription initiation site is determined via different steps, including chromatin remodeling (26). Imamura et al. (27) showed that the chromatin structure-remodeling complex (RSC) subunit Rsc2, a component of the ATP-dependent RSC, physically interacts with the Hap complex and regulates mitochondrial functions. As Fig. 3F indicates, the ⌬rsc2 strain showed a loss in the increase in mitochondrial CRS1 mRNA, as did the disrupted mutants of the HAP gene. We then tested the growth in medium containing glucose or glycerol (Fig. 3G). All strains showed almost normal growth in glucose medium. The ⌬N13-GFP and ⌬hap2 strains, however, exhibited a complete loss of growth in glycerol medium. Also, growth of the ⌬hap-BS and ⌬rsc2 strains was much slower than that of the WT-GFP strain. These results suggest that Hap and RSC cooperate to determine the transcription start site of CRS1, which is essential for mitochondrial energy metabolism and biogenesis.

Discussion
Yeast has a predominantly fermentative metabolism. When grown in medium containing glucose as the carbon source, yeast cells repress their respiratory metabolism to a point where all glucose has been consumed, which leaves only ethanol as a carbon source. To utilize ethanol, yeast cells reprogram their metabolism, a phase known as the diauxic shift (20 -22). During the diauxic shift, the cells regulate mitochondrial bioenergetics The schematic on the right shows the genome structures of WT-GFP and the N-terminal truncated mutant (⌬N13-GFP). DIC, differential interference contrast; Mito, mitochondria. B, Western blot analysis of Crs1 localization in WT-GFP and ⌬N13-GFP. WT-GFP and ⌬N13-GFP were grown in SG to log phase, and the whole (W), cytosolic (C), and mitochondrial (M) fractions were isolated. Protein samples (10 g) were prepared for detection of Crs1-GFP, Pgk1, and Por1. C, Western blot analysis of the cell extracts from WT-GFP and ⌬N13-GFP. Protein samples (10 g) were prepared for detection of Cox2, subunit II of cytochrome c oxidase (a mitochondrial genome-encoded gene), and Por1 (a nuclearly encoded gene). D, growth of WT-GFP and ⌬N13-GFP in glucose or glycerol medium. Cells were grown in glucose medium and then spotted on glucose or glycerol medium.

Mitochondrial yeast cysteinyl-tRNA synthetase and bioenergy
and biogenesis-associated genes and catabolize ethanol via the tricarboxylic acid cycle and oxidative phosphorylation in mitochondria. Some of the genes regulated during the diauxic shift contain mitochondrial ARSs because all mitochondrial genome-encoded proteins are involved in oxidative phosphorylation. Several researchers have proposed that the Hap complex is a key transcription factor involved in the diauxic shift and that it regulates the expression of many genes encoding proteins involved in energy production in mitochondria (28 -30). The Hap complex, known as the CCAAT-binding factor, consists of four components: Hap2, Hap3, Hap4, and Hap5. Formation of the Hap3 and Hap5 heterodimer creates a scaffold for Hap2 association that results in formation of the Hap2/Hap3/Hap5 heterotrimer, which can bind to CCAAT sequences on promoters (31). Hap4 is the activator of the complex. The absence of any subunits of the Hap complex leads to defects in the diauxic shift and growth in nonfermentable medium (i.e. ethanol or glycerol) (31, 32). Therefore, the Hap complex is the master regulator of mitochondrial biogenesis.
In this study, we investigated a mechanism for the generation of a mitochondrial isoform of yeast CysRS (Crs1). This study indicates that the single gene CRS1 expresses two groups of mRNAs through alternative transcription starts. These mRNAs encode two alternative in-frame initiation codons. The mitochondrial Crs1 is translated from the first initiation AUG codon on the long mRNA (mitochondrial mRNA) of CRS1, whereas the cytosolic form is translated from the second in-frame AUG codon on the short mRNA (Fig. 4). The mitochondrial Crs1 therefore has the same polypeptide sequences as the cytosolic Crs1, except for a 13-amino-acid sequence that functions as the MTS at the N terminus. We also found that Hap and RSC likely interact with each other and regulate the transcription start sites of CRS1, which depends on the energy metabolic demand in mitochondria. Fig. 1D shows that the CRS1 expressed from a plasmid with a defined promoter (the ADH1 promoter) starting upstream of the first ATG produces only the mitochondrial isoform, not the cytosol isoform; that is, the ADH1 promoter cannot transcribe . E, ChIP with the CRS1 promoter. Cells with the HA tag inserted into the 3Ј site of HAP2 in the genome were grown in glucose (YPD) or glycerol (YPGly) medium and harvested at L and LS. Binding of Hap2 to the promoter of CRS1 was determined by means of a ChIP assay with HA antibody. Input represents PCR results before immunoprecipitation (IP). F, percentage of mitochondrial CRS1 mRNA in RSC2 mutants. WT and ⌬rsc2 cells were grown in glucose medium (YPD) and harvested at L, ES, and LS. The percentage of mitochondrial CRS1 mRNA was determined using qPCR with total RNA. Results are presented as means Ϯ S.D. (n ϭ 5). Statistical significance was determined by one-way ANOVA with Tukey's test. *, p Ͻ 0.05 (versus the L result); #, p Ͻ 0.05 (versus the ES result). G, growth of WT-GFP, ⌬N13-GFP, ⌬hap2, ⌬hap-BS, and ⌬rsc2 in glucose (YPD) or glycerol (YPGly) medium. Cells were grown in glucose medium and spotted on glucose or glycerol medium.

Mitochondrial yeast cysteinyl-tRNA synthetase and bioenergy
the short mRNA of CRS1. The CRS1 promoter may thus have a particular function that regulates the expression of dual isoforms. In fact, our research showed that the Hap complex is important for the expression of mitochondrial mRNA of CRS1. The Hap complex is well-known to be a key transcription factor involved in the diauxic shift and thereby controls the expression of many genes, which, in turn, may regulate the proteins that mediate energy production in mitochondria (20 -22). Apart from the fact that Crs1 is essential for the translation system in yeast mitochondria, its unique moonlighting function as a CPERS is understood better in view of the energy metabolism as described in this study, which revealed that the Hap complex and Rsc2 are important for expression of the mitochondrial mRNA of CRS1. The RSC functions to rearrange chromatin from a condensed state to a transcriptionally accessible state, which allows gene expression (33). We therefore propose, for the first time, a regulation mechanism operated by the Hap complex and Rsc2 that involves chromatin remodeling, which may depend on the demands of mitochondrial energy metabolism. For example, the Hap complex has been suggested to be activated by binding directly to Rsc2 (27), which may induce up-regulation of the promoter activity for mitochondrial Crs1 expression. This activation process may be mediated by the chromatin remodeling activity of the RSC complex containing Rsc2, which may cause a structural alteration of chromatin from heterochromatin to euchromatin and result in the induction of mitochondrial CRS1 mRNA.
We found that CRS1 encodes both cytoplasmic and the mitochondrial enzymes via alternative use of two in-frame translation initiators. The same transcriptional strategy is also used by yeasts for other single genes that encode both cytoplasmic and mitochondrial proteins. Examples include at least three yeast tRNA-processing enzymes encoded by TRM1, MOD5, and CCA1 and two aminoacyl tRNA-synthetases encoded by HIS1 and VAS1 (14 -16, 34). Each of these genes contains more than one in-frame ATG codon and encodes two or more protein products localized in distinct compartments (i.e. the cytosol and mitochondria). Because all of these genes are somehow involved in the translation system, they are likely to be regulated by the Hap complex and Rsc2. In fact, the sequence upstream of TRM1 and VAS1 contains the Hap complexbinding site, as found by searching the YEASTRACT database (http://www.yeastract.com) 3 (25). This study may therefore warrant additional investigation to obtain a better understanding of alternative gene expression with dual localization of ARSs and mitochondrial proteins in general for other organisms, such as plants.

Yeast strains, medium, and plasmids
Table S1 summarizes the yeast strains (S. cerevisiae BY4742), and Table S2 lists the primers used in this study. The growth media used were yeast extract-peptone-dextrose (YPD), yeast extract-peptone-glycerol (YPGly), synthetic complete (SC), and synthetic complete with galactose (SG) (see supplemental experimental procedures). To construct a chromosomally GFP-tagged CRS1 (YNL247W) strain (WT-GFP), an integration cassette containing a C-terminally positioned GFP tag was amplified by PCR (35). To construct a mutant (⌬N13-GFP) that chromosomally expresses N-terminal 13-residue (39 bp) truncated CRS1 and a strain (⌬hap2-BD) lacking the Hap2-binding site (CCAAT, chromosome XIV 182074_182078) on the CRS1 promoter, the CRISPR-Cas9 system was used (36). For the construction of ⌬hap2-, ⌬mig2-, ⌬rtg1-, ⌬hap3-, ⌬hap4-, and ⌬hap5-disrupted strains, gene-specific deletion cassettes were prepared from the BY4741-derived deletion library (EUROSCARF) by using PCR. To perform the ChIP assay for Hap2, an HA tag-inserted strain (Y800 HAP2-HA) was purchased from Horizon Discovery. All plasmids used in this study were constructed using Gateway technology (Thermo Fisher Scientific, Rockland, IL). The codon-modified CRS1 (Fig. S4) was designed using GeneOptimizer expert software (37) to keep the plasmid containing the CRS1 sequence in Escherichia Figure 4. Dual targeting mechanism of Crs1. CRS1 is transcribed from a single gene with different transcription start sites, which leads to the creation of mRNAs of different lengths: long and short. The long mRNA harboring the first upstream AUG is translated to the Crs1 protein with the MTS, which transports Crs1 to mitochondria. The short mRNA lacking the first AUG is translated from the second AUG to the Crs1 protein without the MTS. This type of Crs1 remains in the cytosol. The need for a specific mitochondrial function governs whether the Hap complex binds to the CCAAT sequence on the CRS1 promoter, which regulates the expression of the long (mitochondrial) mRNA.

Mitochondrial yeast cysteinyl-tRNA synthetase and bioenergy
coli stable. The detailed procedures are described in the supplemental experimental procedures.

Fluorescence microscopy images
WT-GFP and ⌬N13-GFP cells were grown to early stationary phase in filter-sterilized YPD medium at 30°C. WT cells harboring pCRS1full-GFP or pCRS1⌬N13-GFP were grown to early stationary phase in filter-sterilized SC medium without uracil at 30°C. After mitochondrial staining (described below), cells were harvested, washed with PBS, and viewed with a Nikon EZ-C1 confocal laser microscope (Tokyo, Japan).

Mitochondrial isolation
Cells were grown in YPGly or SG with or without uracil to log phase, and crude mitochondrial fractions were isolated as described previously (39). The soluble fractions obtained by this process were filtered through a 0.22-mm filter and used as cytosolic fractions for Western blot assays. For isolation of purified mitochondrial fractions, the crude fractions were layered onto an OptiPrep (Axis-Shield, Oslo, Norway) gradient (a discontinuous gradient of equal volumes of 2%, 8%, 14%, 20%, and 25% iodixanol) in an isotonic buffer (0.6 M sorbitol and 20 mM HEPES-KOH (pH 7.4)). The OptiPrep gradient was centrifuged at 12,000 ϫ g for 2 h, and then the purified mitochondrial fractions were fractionated (40).

Determination of transcription start sites
Transcription start sites of CRS1 were determined via 5Ј-RACE. WT cells were grown to early stationary phase in YPD medium at 30°C. Total RNA was extracted by using TRI reagent with Direct-zol RNA MicroPrep (Zymo Research, Irvine, CA), and poly(A) ϩ RNA was isolated from the total RNA using the Oligotex-dT30 Super mRNA Purification Kit (Takara Bio) according to the manufacturer's instructions. To determine the transcription start sites, circular first-strand cDNA-mediated 5Ј-RACE was employed with poly(A) ϩ RNA using the 5Ј-full RACE core set (Takara Bio) and the following primers: YNL247W RT primer with 5Ј-phosphate for genespecific reverse transcription, YNL247W S1 and YNL247W A2 for the first round of PCR, and YNL247W A1 and YNL247W S2 for the second round of PCR. The second PCR products were cloned into the pTA2 vector (TOYOBO), and 150 sequences were determined.

Growth test
Cells were grown for 2 days in YPD medium and diluted to an optical density of 1.0 with water. Aliquots of 2 l of 10-fold serial dilutions were spotted on YPD or YPGly plates and incubated for 3-4 days at 30°C.

Detection of mitochondrial CRS1 mRNA
Cultured cells were disrupted using the Multi-Beads Shocker (YASUI Kikai, Osaka, Japan) with 0.5-mm glass beads, and total RNA was extracted with the NucleoSpin RNA Plus Kit (Takara Bio) according to the manufacturer's instructions. cDNA was synthesized from total RNA with the PrimeScript RT Reagent Kit (Takara Bio). The relative abundance of mitochondrial CRS1 mRNAs in total CRS1 mRNA was quantified by means of qPCR with the CFX Connect Real-Time System (Bio-Rad) and SsoAdvanced Universal SYBR Green Supermix (Bio-Rad). The following primer sets were used in this analysis (Fig. S3): YNL247W qPCR long form forward and YNL247W qPCR long/short reverse for detection of mitochondrial CRS1 mRNAs (PCR efficiency 98.6%) and YNL247W qPCR short form forward and YNL247W qPCR long/short reverse for detection of total CRS1 mRNAs (PCR efficiency 97.5%). The following PCR protocol was used: 95°C for 4 min, followed by 40 cycles of denaturation at 95°C for 15 s and annealing/extension at 60°C for 30 s. The values of the threshold cycle number (Ct) of the total CRS1 mRNAs and the mitochondrial CRS1 mRNAs were determined for each separate qPCR run. The ⌬Ct (Ct (total CRS1 mRNAs) Ϫ Ct (mitochondrial CRS1 mRNAs)) represents the relative abundance. The quantitative results were expressed as the percentage of mitochondrial CRS1 mRNAs by 2 ⌬Ct .

ChIP assay
The Y800 HAP2-HA strain was cultured in YPD or YPGly medium, and the ChIP assay was performed with anti-HA magnetic beads (Thermo Fisher Scientific) as described by Dedon et al. (42).

Statistical analysis
One-way analysis of variance (ANOVA) with Tukey's test was used for multiple comparisons with GraphPad Prism 7 (GraphPad Software). p Ͻ 0.05 was accepted as the level of statistical significance.