The autism-linked UBE3A T485A mutant E3 ubiquitin ligase activates the Wnt/β-catenin pathway by inhibiting the proteasome

Supplemental Data

  • Supplemental data (.pdf, 4.8 MB) - Supplemental data
  • Supplemental Table 1 (.xlsx, 15 KB) - Table S1. High confidence UBE3A interacting proteins identified by mass spectrometry. We identified high confidence interacting proteins from (24) based on the following criteria: 1) proteins must have a normalized weighted D (NWD) score greater than or equal to 1 in replicate experiments, 2) the interaction must be specific to UBE3A and not NEDD4 (negative control).
  • Supplemental Table 2 (.xlsx, 129 KB) - Table S2. Identification of negative regulators of Wnt signaling by genome-wide siRNA screening in HEK293T cells. Genes that, when knocked down, enhanced Wnt reporter gene expression by at least 50% (p <0.05) from (39) are shown.
  • Supplemental Table 3 (.xlsx, 47 KB) - Table S3. UBE3AT485A and UBE3AT485E interacting proteins identified by mass spectrometry in HEK293T cells.
  • Supplemental Table 4 (.xlsx, 209 KB) - Table S4. Putative UBE3A substrates identified by SILAC and ubiquitin remnant immunoaffinity profiling in HEK293T cells. The proteins listed exhibited at least a 20% enrichment in ubiquitination in cells expressing UBE3AT485A as compared to cells expressing UBE3AT485E. Core proteasome subunits are highlighted.
  • Supplemental Table 5 (.xlsx, 12 KB) - Table S5. Rescue of UBE3A-dependent Wnt activation by proteasomal subunits.
  • Supplemental Table 6 (.xlsx, 11 KB) - Table S6. BAR reporter responses in HEK293T cells upon knockdown of proteasome subunits.

This Article

  1. First Published on May 30, 2017, doi: 10.1074/jbc.M117.788448 JBC May 30, 2017
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