Journal of Biological Chemistry current issue http://www.jbc.org Journal of Biological Chemistry RSS feed -- current issue 1083-351X Sep 4 2020 12:00:00:000AM Journal of Biological Chemistry 0021-9258 Journal of Biological Chemistry http://www.jbc.org/icons/banner/title.gif http://www.jbc.org <![CDATA[Targeting all transforming growth factor-{beta} isoforms with an Fc chimeric receptor impairs tumor growth and angiogenesis of oral squamous cell cancer [Signal Transduction]]]> http://www.jbc.org/content/295/36/12559.short?rss=1 2020-09-04T00:05:37-07:00 info:doi/10.1074/jbc.RA120.012492 hwp:master-id:jbc;RA120.012492 ASBMB 2020-09-04 Editors' Picks Signal Transduction 295 36 12559 12572 <![CDATA[A polysaccharide extract from the medicinal plant Maidong inhibits the IKK-NF-{kappa}B pathway and IL-1{beta}-induced islet inflammation and increases insulin secretion [Metabolism]]]> http://www.jbc.org/content/295/36/12573.short?rss=1 2020-09-04T00:05:37-07:00 info:doi/10.1074/jbc.RA120.014357 hwp:master-id:jbc;RA120.014357 ASBMB 2020-09-04 Cell Biology Metabolism 295 36 12573 12587 <![CDATA[A cryptic sequence targets the adhesion complex scaffold ANKS4B to apical microvilli to promote enterocyte brush border assembly [Developmental Biology]]]> http://www.jbc.org/content/295/36/12588.short?rss=1 2020-09-04T00:05:37-07:00 info:doi/10.1074/jbc.RA120.013790 hwp:master-id:jbc;RA120.013790 ASBMB 2020-09-04 Cell Biology Developmental Biology 295 36 12588 12604 <![CDATA[Cathepsin A contributes to left ventricular remodeling by degrading extracellular superoxide dismutase in mice [Molecular Bases of Disease]]]> http://www.jbc.org/content/295/36/12605.short?rss=1 2020-09-04T00:05:37-07:00 info:doi/10.1074/jbc.RA120.013488 hwp:master-id:jbc;RA120.013488 ASBMB 2020-09-04 Molecular Bases of Disease 295 36 12605 12617 <![CDATA[Spectroscopic evidence for direct flavin-flavin contact in a bifurcating electron transfer flavoprotein [Bioenergetics]]]> http://www.jbc.org/content/295/36/12618.short?rss=1 2020-09-04T00:05:37-07:00 info:doi/10.1074/jbc.RA120.013174 hwp:master-id:jbc;RA120.013174 ASBMB 2020-09-04 Enzymology Bioenergetics 295 36 12618 12634 <![CDATA[The first DEP domain of the RhoGEF P-Rex1 autoinhibits activity and contributes to membrane binding [Protein Structure and Folding]]]> http://www.jbc.org/content/295/36/12635.short?rss=1 2020-09-04T00:05:37-07:00 info:doi/10.1074/jbc.RA120.014534 hwp:master-id:jbc;RA120.014534 ASBMB 2020-09-04 Signal Transduction Protein Structure and Folding 295 36 12635 12647 <![CDATA[Glycan analysis of human neutrophil granules implicates a maturation-dependent glycosylation machinery [Glycobiology and Extracellular Matrices]]]> http://www.jbc.org/content/295/36/12648.short?rss=1 2020-09-04T00:05:37-07:00 info:doi/10.1074/jbc.RA120.014011 hwp:master-id:jbc;RA120.014011 ASBMB 2020-09-04 Glycobiology and Extracellular Matrices 295 36 12648 12660 <![CDATA[Ubiquitin ligase SMURF2 enhances epidermal growth factor receptor stability and tyrosine-kinase inhibitor resistance [Cell Biology]]]> http://www.jbc.org/content/295/36/12661.short?rss=1 2020-09-04T00:05:37-07:00 info:doi/10.1074/jbc.RA120.013519 hwp:master-id:jbc;RA120.013519 ASBMB 2020-09-04 Protein Synthesis and Degradation Cell Biology 295 36 12661 12673 <![CDATA[A comprehensive analysis of Rab GTPases reveals a role for Rab34 in serum starvation-induced primary ciliogenesis [Membrane Biology]]]> http://www.jbc.org/content/295/36/12674.short?rss=1 2020-09-04T00:05:37-07:00 info:doi/10.1074/jbc.RA119.012233 hwp:master-id:jbc;RA119.012233 ASBMB 2020-09-04 Cell Biology Membrane Biology 295 36 12674 12685 <![CDATA[Intracellular autoactivation of TMPRSS11A, an airway epithelial transmembrane serine protease [Enzymology]]]> http://www.jbc.org/content/295/36/12686.short?rss=1 2020-09-04T00:05:37-07:00 info:doi/10.1074/jbc.RA120.014525 hwp:master-id:jbc;RA120.014525 ASBMB 2020-09-04 Enzymology 295 36 12686 12696 <![CDATA[Peroxidasin mediates bromination of tyrosine residues in the extracellular matrix [Cell Biology]]]> http://www.jbc.org/content/295/36/12697.short?rss=1 2020-09-04T00:05:37-07:00 info:doi/10.1074/jbc.RA120.014504 hwp:master-id:jbc;RA120.014504 ASBMB 2020-09-04 Enzymology Cell Biology 295 36 12697 12705 <![CDATA[PE5-PPE4-EspG3 heterotrimer structure from mycobacterial ESX-3 secretion system gives insight into cognate substrate recognition by ESX systems [Microbiology]]]> http://www.jbc.org/content/295/36/12706.short?rss=1 2020-09-04T00:05:37-07:00 info:doi/10.1074/jbc.RA120.012698 hwp:master-id:jbc;RA120.012698 ASBMB 2020-09-04 Protein Structure and Folding Microbiology 295 36 12706 12715 <![CDATA[Caspr2 interacts with type 1 inositol 1,4,5-trisphosphate receptor in the developing cerebellum and regulates Purkinje cell morphology [Molecular Bases of Disease]]]> http://www.jbc.org/content/295/36/12716.short?rss=1 2020-09-04T00:05:37-07:00 info:doi/10.1074/jbc.RA120.012655 hwp:master-id:jbc;RA120.012655 ASBMB 2020-09-04 Neurobiology Molecular Bases of Disease 295 36 12716 12726 <![CDATA[Ozone-derived oxysterols impair lung macrophage phagocytosis via adduction of some phagocytosis receptors [Cell Biology]]]> http://www.jbc.org/content/295/36/12727.short?rss=1 2020-09-04T00:05:37-07:00 info:doi/10.1074/jbc.RA120.013699 hwp:master-id:jbc;RA120.013699 ASBMB 2020-09-04 Cell Biology 295 36 12727 12738 <![CDATA[Inhibitors of a Na+-pumping NADH-ubiquinone oxidoreductase play multiple roles to block enzyme function [Enzymology]]]> http://www.jbc.org/content/295/36/12739.short?rss=1 2020-09-04T00:05:37-07:00 info:doi/10.1074/jbc.RA120.014229 hwp:master-id:jbc;RA120.014229 ASBMB 2020-09-04 Bioenergetics Enzymology 295 36 12739 12754 <![CDATA[Structure of a collagen VI {alpha}3 chain VWA domain array: adaptability and functional implications of myopathy causing mutations [Glycobiology and Extracellular Matrices]]]> http://www.jbc.org/content/295/36/12755.short?rss=1 2020-09-04T00:05:37-07:00 info:doi/10.1074/jbc.RA120.014865 hwp:master-id:jbc;RA120.014865 ASBMB 2020-09-04 Protein Structure and Folding Glycobiology and Extracellular Matrices 295 36 12755 12771 <![CDATA[Characterization of the endoplasmic reticulum-resident peroxidases GPx7 and GPx8 shows the higher oxidative activity of GPx7 and its linkage to oxidative protein folding [Protein Structure and Folding]]]> http://www.jbc.org/content/295/36/12772.short?rss=1 2020-09-04T00:05:37-07:00 info:doi/10.1074/jbc.RA120.013607 hwp:master-id:jbc;RA120.013607 ASBMB 2020-09-04 Enzymology Protein Structure and Folding 295 36 12772 12785 <![CDATA[Brain cell somatic gene recombination and its phylogenetic foundations [Genomics and Proteomics]]]> http://www.jbc.org/content/295/36/12786.short?rss=1 2020-09-04T00:05:37-07:00 info:doi/10.1074/jbc.REV120.009192 hwp:master-id:jbc;REV120.009192 ASBMB 2020-09-04 JBC Reviews DNA and Chromosomes Genomics and Proteomics 295 36 12786 12795 <![CDATA[Regulation of eukaryotic translation initiation factor 6 dynamics through multisite phosphorylation by GSK3 [Protein Synthesis and Degradation]]]> http://www.jbc.org/content/295/36/12796.short?rss=1 2020-09-04T00:05:37-07:00 info:doi/10.1074/jbc.RA120.013324 hwp:master-id:jbc;RA120.013324 ASBMB 2020-09-04 Cell Biology Protein Synthesis and Degradation 295 36 12796 12813 <![CDATA[Identification of an anti-SARS-CoV-2 receptor-binding domain-directed human monoclonal antibody from a naive semisynthetic library [Methods and Resources]]]> http://www.jbc.org/content/295/36/12814.short?rss=1 2020-09-04T00:05:37-07:00 info:doi/10.1074/jbc.AC120.014918 hwp:master-id:jbc;AC120.014918 ASBMB 2020-09-04 Accelerated Communications Immunology Methods and Resources 295 36 12814 12821 <![CDATA[The tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex [Membrane Biology]]]> http://www.jbc.org/content/295/36/12822.short?rss=1 100 substrates, including Notch, amyloid precursor protein, cadherins, growth factors, and chemokines. ADAM10 has been recently proposed to function as six distinct scissors with different substrates, depending on its association with one of six regulatory tetraspanins, termed TspanC8s. However, it remains unclear to what degree ADAM10 function critically depends on a TspanC8 partner, and a lack of monoclonal antibodies specific for most TspanC8s has hindered investigation of this question. To address this knowledge gap, here we designed an immunogen to generate the first monoclonal antibodies targeting Tspan15, a model TspanC8. The immunogen was created in an ADAM10-knockout mouse cell line stably overexpressing human Tspan15, because we hypothesized that expression in this cell line would expose epitopes that are normally blocked by ADAM10. Following immunization of mice, this immunogen strategy generated four Tspan15 antibodies. Using these antibodies, we show that endogenous Tspan15 and ADAM10 co-localize on the cell surface, that ADAM10 is the principal Tspan15-interacting protein, that endogenous Tspan15 expression requires ADAM10 in cell lines and primary cells, and that a synthetic ADAM10/Tspan15 fusion protein is a functional scissor. Furthermore, two of the four antibodies impaired ADAM10/Tspan15 activity. These findings suggest that Tspan15 directly interacts with ADAM10 in a functional scissor complex.]]> 2020-09-04T00:05:37-07:00 info:doi/10.1074/jbc.RA120.012601 hwp:master-id:jbc;RA120.012601 ASBMB 2020-09-04 Cell Biology Membrane Biology 295 36 12822 12839