Searching journal content for yeast (as phrase) in full text.

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  1. Cell Biology - Membrane Biology:
    • Jennifer Virginia Roche,
    • Sabeen Survery,
    • Stefan Kreida,
    • Veronika Nesverova,
    • Henry Ampah-Korsah,
    • Maria Gourdon,
    • Peter M.T. Deen,
    • and Susanna Toernroth-Horsefield
    Phosphorylation of human aquaporin 2 (AQP2) allosterically controls its interaction with the lysosomal trafficking protein LIP5 J. Biol. Chem. jbc.M117.788364. doi:10.1074/jbc.M117.788364
    ...in Pichia pastoris cDNAs coding for wild-type human AQP2, AQP2 phosphomimicking mutants (S256E, S261E, S264E, T269E) and AQP2 truncated after P242 (AQP2P242) were cloned into yeast expression vectors, overproduced in the yeast Pichia pastoris, and purified. Since Pichia is known to be able ~~~
  2. Cell Biology - Enzymology:
    • Andrew S Murray,
    • Fausto A Varela,
    • Thomas E Hyland,
    • Andrew J Schoenbeck,
    • Jordan M White,
    • Lauren M Tanabe,
    • Sokol V Todi,
    • and Karin List
    Phosphorylation of the type II transmembrane serine protease, TMPRSS13 in Hepatocyte Growth Factor Activator Inhibitor-1 and 2-mediated cell surface localization J. Biol. Chem. jbc.M117.775999. doi:10.1074/jbc.M117.775999
    ...expression system which utilizes the intracellular yeast protease KEX2 was employed. The KEX2 transmembrane serine protease belongs to the subtilisin-like, pro-protein convertase family with specificity for cleavage after paired basic amino acids and is localized in the late Golgi compartment. By cloning ~~~
  3. Protein Structure and Folding - Molecular Bases of Disease:
    • Gina M Moriarty,
    • Michael P Olson,
    • Tamr B Atieh,
    • Maria K Janowska,
    • Sagar D Khare,
    • and Jean Baum
    A pH Dependent Switch Promotes β-Synuclein Fibril Formation via Glutamate Residues J. Biol. Chem. jbc.M117.780528. doi:10.1074/jbc.M117.780528
    ...associated with S are linked with defects in ER-Golgi associated proteins in yeast, which is a mildly acidic subcellular environment with pH values near our experimental pH of 5.8. There is also an emerging role for endolysosomal dysfunction in PD, particularly in relation to recent reports of Gaucher ~~~
  4. Protein Synthesis and Degradation - Signal Transduction:
    • Jeffrey A. Willy,
    • Sara K. Young,
    • Amber L. Mosley,
    • Samer Gawrieh,
    • James L. Stevens,
    • Howard C. Masuoka,
    • and Ronald C. Wek
    Function of Inhibitor of Brutons Tyrosine Kinase Isoform α (IBTKα) in Nonalcoholic Steatohepatitis links Autophagy and the Unfolded Protein Response J. Biol. Chem. jbc.M117.799304. doi:10.1074/jbc.M117.799304
    ...yeast ~~~
  5. Bioenergetics - Methods and Resources:
    • Edward T. Chouchani,
    • Andrew M. James,
    • Carmen Methner,
    • Victoria R. Pell,
    • Tracy A. Prime,
    • Brian K. Erikson,
    • Marleen Forkink,
    • Gigi Y. Lau,
    • Thomas P. Bright,
    • Katja E. Menger,
    • Ian M. Fearnley,
    • Thomas Krieg,
    • and Michael P. Murphy
    Identification and Quantification of Protein S-nitrosation by Nitrite in the Mouse Heart during Ischemia J. Biol. Chem. jbc.M117.798744. doi:10.1074/jbc.M117.798744
    ...., Harding, M., Monne, M., Slotboom, D. J., and Kunji, E. R. (2007) The yeast mitochondrial ADP/ATP carrier functions as a monomer in mitochondrial membranes. Proc. Natl. Acad. Sci. USA 104, 10830-10834 37. McStay, G. P., Clarke, S. J., and Halestrap, A. P. (2002) Role of critical thiol groups on the matrix ~~~
  6. Microbiology - Bioenergetics:
    • Laurent Loiseau,
    • Cameron Fyfe,
    • Laurent Aussel,
    • Mahmoud Hajj Chehade,
    • Sara B. Hernández,
    • Bruno Faivre,
    • Djemel Hamdane,
    • Caroline Mellot-Draznieks,
    • Bérengère Rascalou,
    • Ludovic Pelosi,
    • Christophe Velours,
    • David Cornu,
    • Murielle Lombard,
    • Josep Casadesús,
    • Fabien Pierrel,
    • Marc Fontecave,
    • and Frédéric Barras
    The UbiK protein is an accessory factor necessary for bacterial ubiquinone (UQ) biosynthesis and forms a complex with the UQ biogenesis factor UbiJ J. Biol. Chem. 2017 292: 11937-. doi:10.1074/jbc.M117.789164
    ...) antibodies, respectively. Thus, biochemical analyses allowed us to confirm that UbiK interacts with UbiJ. Last, yeast two-hybrid-based tests were run as they report on direct interactions and might eventually allow to pinpoint zones of interaction. Evidence of an UbiKUbiJ direct interaction was found ~~~
  7. Protein Synthesis and Degradation - Cell Biology:
    • Pattarawut Sopha,
    • Hong Yu Ren,
    • Diane E. Grove,
    • and Douglas M. Cyr
    Endoplasmic reticulum stress–induced degradation of DNAJB12 stimulates BOK accumulation and primes cancer cells for apoptosis J. Biol. Chem. 2017 292: 11792-. doi:10.1074/jbc.M117.785113
    .... Chem. (2017) 292(28) 1179211803 11793 DUF1997 plays in JB12 function, but it is just110 amino acid residues long (Fig. 2A) and is conserved in ER transmembrane Hsp40s that are expressed in organisms from yeast to man (7, 20). JB12 and JB14 both contain a DUF1977 domain, but the stability of JB12 ~~~
  8. Protein Structure and Folding - Molecular Biophysics:
    • Xiang Li,
    • Marie Anderson,
    • Delphine Collin,
    • Ingo Muegge,
    • John Wan,
    • Debra Brennan,
    • Stanley Kugler,
    • Donna Terenzio,
    • Charles Kennedy,
    • Siqi Lin,
    • Mark E. Labadia,
    • Brian Cook,
    • Robert Hughes,
    • and Neil A. Farrow
    Structural studies unravel the active conformation of apo RORγt nuclear receptor and a common inverse agonism of two diverse classes of RORγt inhibitors J. Biol. Chem. 2017 292: 11618-. doi:10.1074/jbc.M117.789024
    ...). RORt-Gal4 reporter gene assay HEK293T cells were co-transfected with a plasmid pBIND containing the chimera of the DNA-binding domain of the yeast Gal4 protein and the ligand-binding domain of human RORt (Gal4DBD-hRORt LBD), along with the luciferase reporter plasmid pGL4.31 (luc2P/GAL4UAS ~~~
  9. DNA and Chromosomes - Molecular Biophysics:
    • Chu Jian Ma,
    • Youngho Kwon,
    • Patrick Sung,
    • and Eric C. Greene
    Human RAD52 interactions with replication protein A and the RAD51 presynaptic complex J. Biol. Chem. 2017 292: 11702-. doi:10.1074/jbc.M117.794545
    ...-damaging agents (2, 40, 41). Rad52 is highly conserved, and human RAD52 shares many biochemical traits with its yeast counterpart (42, 43). Like yeast Rad52, human RAD52 also forms ring-like structures (4447), binds tightly to ssDNA, and promotes ssDNA annealing (47 51). However, human RAD52 does not have ~~~
  10. Microbiology - Metabolism:
    • Jessica L. Chitty,
    • Kirsten L. Blake,
    • Ross D. Blundell,
    • Y. Q. Andre E. Koh,
    • Merinda Thompson,
    • Avril A. B. Robertson,
    • Mark S. Butler,
    • Matthew A. Cooper,
    • Ulrike Kappler,
    • Simon J. Williams,
    • Bostjan Kobe,
    • and James A. Fraser
    Cryptococcus neoformans ADS lyase is an enzyme essential for virulence whose crystal structure reveals features exploitable in antifungal drug design J. Biol. Chem. 2017 292: 11829-. doi:10.1074/jbc.M117.787994
    ...The abbreviations used are: ADS, adenylosuccinate; SAICAR, succinylaminoimidazole carboxamide riboside; AICAR, aminoimidazole carboxamide riboside; SEC, size exclusion chromatography; MALLS, multi-angle laser light scattering; YNB, yeast nitrogen base; L-DOPA, L-3,4-dihydroxyphenylalanine; BisTris, 2-[bis(2 ~~~
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