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DNA and Chromosomes
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- DNA and ChromosomesOpen Access
Pif1, RPA, and FEN1 modulate the ability of DNA polymerase δ to overcome protein barriers during DNA synthesis
Journal of Biological ChemistryVol. 295Issue 47p15883–15891Published online: September 10, 2020- Melanie A. Sparks
- Peter M. Burgers
- Roberto Galletto
Cited in Scopus: 10Successful DNA replication requires carefully regulated mechanisms to overcome numerous obstacles that naturally occur throughout chromosomal DNA. Scattered across the genome are tightly bound proteins, such as transcription factors and nucleosomes, that are necessary for cell function, but that also have the potential to impede timely DNA replication. Using biochemically reconstituted systems, we show that two transcription factors, yeast Reb1 and Tbf1, and a tightly positioned nucleosome, are strong blocks to the strand displacement DNA synthesis activity of DNA polymerase δ.