DNA and Chromosomes
Unifying the DNA End-processing Roles of the Artemis Nuclease: KU-DEPENDENT ARTEMIS RESECTION AT BLUNT DNA ENDS
Background: Artemis is a nuclease that is necessary for hairpin opening in V(D)J recombination.Results: Artemis action on blunt DNA ends is dependent on DNA sequence (breathing) and Ku.Conclusion: Breathing of blunt DNA ends into a transient ss/dsDNA boundary is needed for Artemis action.Significance: Unification of Artemis nuclease action explains the features of NHEJ and V(D)J recombination.
