Enzymology
The GH130 Family of Mannoside Phosphorylases Contains Glycoside Hydrolases That Target β-1,2-Mannosidic Linkages in Candida Mannan
Background: A cohort of a family of mannose phosphorylases lack phosphate binding residues, suggesting that they display non-phosphorylase activities.Results: The non-phosphorylase enzymes were shown to be β-mannosidases.Conclusion: Replacing basic phosphate binding residues with carboxylic amino acids converts mannoside phosphorylases into glycoside hydrolases.Significance: Functional phylogeny can be used to distinguish between closely related glycan phosphorylases and glycoside hydrolases.
