Enzymology
Multidomain Human Peroxidasin 1 Is a Highly Glycosylated and Stable Homotrimeric High Spin Ferric Peroxidase
Background: Human peroxidasin 1 (hsPxd01) mediates the formation of sulfilimine cross-links within the collagen IV scaffold of basement membranes.Results: Overexpressed hsPxd01 contains covalently linked heme catalytically active for production of hypobromous acid.Conclusion: hsPxd01 has peroxidase-like active site structure but restricted substrate accessibility.Significance: Architecture of hsPxd01 facilitates product release and its interactions with the physiological substrate collagen IV.
