Gene Regulation
Protein Phosphatase PP5 Controls Bone Mass and the Negative Effects of Rosiglitazone on Bone through Reciprocal Regulation of PPARγ (Peroxisome Proliferator-activated Receptor γ) and RUNX2 (Runt-related Transcription Factor 2)
Peroxisome proliferator-activated receptor γ (PPARγ) and runt-related transcription factor 2 (RUNX2) are key regulators of mesenchymal stem cell (MSC) differentiation toward adipocytes and osteoblasts, respectively. Post-translational modifications of these factors determine their activities. Dephosphorylation of PPARγ at Ser-112 is required for its adipocytic activity, whereas phosphorylation of RUNX2 at serine 319 (Ser-319) promotes its osteoblastic activity. Here we show that protein phosphatase 5 (PP5) reciprocally regulates each receptor by targeting each serine.Reactive Oxygen Species (ROS) Mediate p300-dependent STAT1 Protein Interaction with Peroxisome Proliferator-activated Receptor (PPAR)-γ in CD36 Protein Expression and Foam Cell Formation
Background: CD36 plays a role in lipid uptake, foam cell formation, and atherogenesis.Results: 15(S)-HETE induces CD36 expression and foam cell formation by triggering p300-mediated STAT1 acetylation and its interaction with PPARγ.Conclusion: STAT1 and PPARγ interact with each other in CD36 expression and foam cell formation.Significance: 15(S)-HETE appears to play an important role in foam cell formation, a critical event in atherogenesis.
