Signal Transduction
Evaluating the Role of Retinal Membrane Guanylyl Cyclase 1 (RetGC1) Domains in Binding Guanylyl Cyclase-activating Proteins (GCAPs)
Background: GCAP1 and GCAP2 regulate cGMP synthesis by RetGC1 in photoreceptors.Results: GCAPs compete for binding to RetGC1 in biochemical assays and in HEK293 cells co-expressing fluorescently labeled GCAPs with different forms of RetGC1.Conclusion: The GCAP1 and GCAP2 binding site(s) overlaps within the kinase homology and/or dimerization domains of RetGC1.Significance: RetGC1 and GCAPs contribute to normal vision and congenital blindness in humans.
