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Human plasma carboxypeptidase N has been purified 2,600-fold from pooled, outdated plasma in a 30% yield. Isolation was accomplished by chromatography on DEAE-cellulose and on a p-aminobenzoyl-L-arginine-Sepharose 6B affinity column. Carbohydrate accounts for 17% of the weight calculated from its amino acid and carbohydrate composition. The enzyme appears to consist of three subunits of Mr = 83,000, 55,000, and 49,000 and contains a significant amount of bound zinc. Purified enzyme preparations are very sensitive to proteolytic degradation but are stable for at least 3 months at 4 degrees.
REFERENCES
- Biochem. Pharmacol. 1962; 11: 585-592
- Biochem. Pharmacol. 1964; 13: 883-905
- Biochem. Pharmacol. 1967; 16: 1287-1297
- J. Clin. Invest. 1970; 49: 2427-2436
- Biochim. Biophys. Acta. 1974; 365: 344-348
- Arch. Biochem. Biophys. 1975; 170: 132-138
- Biochim. Biophys. Acta. 1971; 251: 203-206
- Biochemistry. 1978; 17: 401-405
- J. Biol. Chem. 1951; 193: 265-275
- Biochemistry. 1962; 1: 1078-1082
- J. Biol. Chem. 1962; 237: 3094-3099
- Arch. Biochem. Biophys. 1968; 123: 336-342
- Biochim. Biophys. Acta. 1961; 48: 139-147
- J. Chromatogr. 1970; 51: 479-489
- Biochemistry. 1975; 14: 4813-4820
- Anal. Biochem. 1964; 60: 15-24
- Methods Enzymol. 1972; 25B: 464-468
- J. Biol. Chem. 1964; 239: 1799-1803
- Can. J. Biochem. 1971; 49: 1055-1061
- Methods Enzymol. 1976; 45B: 257-273
- J. Biol. Chem. 1969; 244: 4406-4412
- Methods Enzymol. 1972; 28B: 54-63
- Anal. Chem. 1958; 30: 1190-1206
- Fed. Proc. 1964; 23: 371
- J. Biol. Chem. 1968; 243: 6281-6283
- J. Biol. Chem. 1963; 238: 235-237
- Anal. Biochem. 1974; 60: 45-50
- Anal. Biochem. 1976; 73: 532-534
- Anal. Biochem. 1969; 29: 433-440
- J. Biol. Chem. 1959; 234: 1971-1975
- Anal. Biochem. 1978; 86 (in press)
- Gottschalk A. 2nd Ed. Glycoproteins. Elsevier, Amsterdam1972: 31-140 (Part A)
- J. Biol. Chem. 1969; 244: 5246-5253
- Biochemistry. 1962; 1: 1078-1082
- Dayhoff M.O. Atlas of Protein Sequence and Structure. 5. National Biomedical Research Foundation, Washington, D.C.1976: 301-310 (Suppl. 2)
- Nature. 1965; 206: 777-779
- Putnam F. 2nd Ed. The Plasma Proteins. 2. Academic Press, New York1975: 163-211
- J. Exp. Med. 1973; 137: 1109-1123
- Hoppe-Seyler's Z. Physiol. Chem. 1976; 357: 867-872
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Published online: June 10, 1978
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© 1978 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.
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