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Research Article| Volume 260, ISSUE 23, P12492-12499, October 15, 1985

Studies on the phosphorylation of myelin basic protein by protein kinase C and adenosine 3‘:5‘-monophosphate-dependent protein kinase.

Open AccessPublished:October 15, 1985DOI:https://doi.org/10.1016/S0021-9258(17)38898-1
Studies on the phosphorylation of myelin basic protein by protein kinase C and adenosine 3‘:5‘-monophosphate-dependent protein kinase.
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      The substrate specificity of protein kinase C was studied and compared with that of cyclic AMP-dependent protein kinase (protein kinase A) by using bovine brain myelin basic protein as a model substrate. This basic protein was phosphorylated at multiple sites by both of these protein kinases. In this analysis, the basic protein was thoroughly phosphorylated in vitro with [gamma-32P]ATP and each protein kinase, and then digested with trypsin. The resulting radioactive phosphopeptides were isolated by gel filtration followed by high performance liquid chromatography on a reverse-phase column. Subsequent amino acid analysis and/or sequential Edman degradation of the purified phosphopeptides, together with the known primary sequence of this protein, revealed that Ser-46 and Ser-151 were specifically phosphorylated by protein kinase C, whereas Thr-34 and Ser-115 were phosphorylated preferentially by protein kinase A. Both kinases reacted with Ser-8, Ser-11, Ser-55, Ser-110, Ser-132, and Ser-161 at various reaction velocities. Contrary to protein kinase A, protein kinase C appears to react preferentially with seryl residues that are located at the amino-terminal side close to lysine or arginine. The seryl residues that are phosphorylated commonly by these two protein kinases have basic amino acids at both the amino- and carboxyl-terminal sides. These results provide some clues to understanding the rationale that these kinases may show different but sometimes similar functions depending on the structure of target phosphate acceptor proteins.

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      Published online: October 15, 1985

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      DOI: https://doi.org/10.1016/S0021-9258(17)38898-1

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      © 1985 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.

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