Molecular cloning and expression of a glycosaminoglycan N-acetylglucosaminyl N-deacetylase/N-sulfotransferase from a heparin-producing cell line.

A. Orellana; C.B. Hirschberg; Z. Wei; S.J. Swiedler; M. Ishihara

doi:10.1016/S0021-9258(17)42164-8

Journal Article| Volume 269, ISSUE 3, P2270-2276, January 21, 1994

Molecular cloning and expression of a glycosaminoglycan N-acetylglucosaminyl N-deacetylase/N-sulfotransferase from a heparin-producing cell line.

A. Orellana
A. Orellana
Affiliations
Department of Biochemistry and Molecular Biology, University of Massachusetts Medical Center, Worcester 01655.
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C.B. Hirschberg
C.B. Hirschberg
Affiliations
Department of Biochemistry and Molecular Biology, University of Massachusetts Medical Center, Worcester 01655.
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Z. Wei
Z. Wei
Affiliations
Department of Biochemistry and Molecular Biology, University of Massachusetts Medical Center, Worcester 01655.
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S.J. Swiedler
S.J. Swiedler
Affiliations
Department of Biochemistry and Molecular Biology, University of Massachusetts Medical Center, Worcester 01655.
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M. Ishihara
M. Ishihara
Affiliations
Department of Biochemistry and Molecular Biology, University of Massachusetts Medical Center, Worcester 01655.
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Open AccessPublished:January 21, 1994DOI:https://doi.org/10.1016/S0021-9258(17)42164-8

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Heparin has a higher content of N-sulfated glucosamine and L-iduronic acid than heparan sulfate. Deacetylation of N-acetylglucosamine followed by N-sulfation may be important steps differentiating the biosynthesis of these glycosaminoglycans. We have cloned, by cross-hybridization with the cDNA from rat liver heparan sulfate N-deacetylase/N-sulfotransferase, a protein from a heparin synthesizing mastocytoma derived cell line called MST. This protein, which has both N-deacetylase/N-sulfotransferase activities, has a predicted amino acid sequence homology of 70% with the above rat liver enzyme and is unique for the following reasons. 1) It was found to be encoded by a 3.8-kilobase mRNA that was unique to heparin-producing cells; an 8.5-kilobase mRNA encoding the rat liver enzymes has been found to occur in all mammalian cells tested on the basis of nucleic acid cross-hybridization; 2) the protein overexpressed in COS cells in its full-length transmembrane form or as a soluble secreted protein A chimera displayed ratios of N-deacetylase to N-sulfotransferase activities that were 4-8-fold higher than that observed for the enzyme found in liver that is involved in the biosynthesis of heparan sulfate. These results suggest that the MST-derived enzyme is probably unique to the production of heparin in mast cells.

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Published online: January 21, 1994

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Molecular cloning and expression of a glycosaminoglycan N-acetylglucosaminyl N-deacetylase/N-sulfotransferase from a heparin-producing cell line.

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