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Cell-free translations of proline-rich protein mRNAs.

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      Treatment of rats with the beta-agonist isoproterenol causes a dramatic increase in a series of proteins rich in proline (proline-rich proteins) in the parotid glands (Muenzer, J., Bildstein, C., Gleason, M., and Carlson, D. M. (1979) J. Biol. Chem. 254, 5623, 5629). These proteins which contain about 43% proline, comprise more than 50% of the total soluble protein of glands of rats treated with isoproterenol for 10 days. Further studies by in vitro translation analysis using the reticulocyte lysate system and labeling with [3H]proline or [35S]methionine show definitive changes in patterns of protein synthesis and proline-rich protein mRNAs are highly elevated in treated animals. Analysis of translation products by sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed (1) very little synthesis of proline-rich proteins from poly(A+) RNA of glands of normal rats, (2) poly(A+) RNA from glands of treated animals synthesize mainly proline-rich proteins, (3) translations with [3H]proline and [35S]methionine give identical labeling patterns from cell-free translations are all precipitated by antibodies to proline-rich proteins. At least six different proline-rich proteins are translated with poly(A+) mRNA from glands of treated animals. Each of these proteins is likely a translation product of a separate, specific mRNA. The dramatic changes in protein synthesis of rat parotid glands in response to isoproterenol treatment suggest to us that the parotid gland of the isoproterenol-treated rat is an excellent model system to study the overall responsiveness of gene expression to catecholamines.

      REFERENCES

        • Regier J.C.
        • Kafatos F.C.
        • Goodfliesh R.
        • Hood L.
        Proc. Natl. Acad. Sci. U. S. A. 1978; 75: 390-394
        • Thireos G.
        • Kafatos F.C.
        Dev. Biol. 1980; 78: 36-46
        • MacDonald R.J.
        • Crerar M.M.
        • Swain W.F.
        • Pictet R.L.
        • Thomas G.
        • Rutter W.J.
        Nature. 1980; 287: 117-122
        • Young R.A.
        • Hagenbuckle O.
        • Schibler U.
        Cell. 1981; 23: 451-458
        • McKnight G.S.
        • Palmiter R.D.
        J. Biol. Chem. 1979; 254: 9050-9058
        • LeMeur M.
        • Glanville N.
        • Mandel J.L.
        • Gerlinger P.
        • Palmiter R.D.
        • Chambon P.
        Cell. 1981; 23: 561-571
        • Selye H.
        • Cantin M.
        • Veilleux R.
        Growth. 1961; 25: 243-248
        • Brown-Grant K.
        Nature. 1961; 191: 1076-1078
        • Muenzer J.
        • Bildstein C.
        • Gleason M.
        • Carlson D.M.
        J. Biol. Chem. 1979; 254: 5623-5628
        • Muenzer J.
        • Bildstein C.
        • Gleason M.
        • Carlson D.M.
        J. Biol. Chem. 1979; 254: 5629-5634
        • Kumarasamy R.
        • Symons R.H.
        Anal. Biochem. 1979; 95: 359-363
        • Laemmli U.K.
        Nature. 1970; 227: 680-685
        • Harding J.D.
        • Przybyla A.E.
        • MacDonald R.J.
        • Pictet R.L.
        • Rutter W.J.
        J. Biol. Chem. 1978; 253: 7531-7537
        • Aviv H.
        • Leder P.
        Proc. Natl. Acad. Sci. U. S. A. 1972; 69: 1408-1412
        • Darnbrough C.
        • Legon S.
        • Hunt T.
        • Jackson R.
        J. Mol. Biol. 1973; 76: 379-403
        • Pelham H.R.B.
        • Jackson R.J.
        Eur. J. Biochem. 1976; 67: 247-256
        • Bonner W.B.
        • Laskey R.A.
        Eur. J. Biochem. 1974; 46: 83-88
        • Kreil G.
        Annu. Rev. Biochem. 1981; 50: 317-348
        • Palmiter R.D.
        • Gagnon J.
        • Ericsson L.H.
        • Walsh K.A.
        J. Biol. Chem. 1977; 252: 6386-6393
        • Furthmayr H.
        • Timpl R.
        Anal. Biochem. 1971; 41: 510-516
        • Tsang B.K.
        • Dixon R.H.
        • Whitfield J.F.
        J. Cell Physiol. 1980; 102: 19-26
        • Butcher F.R.
        • Rudich L.
        • Einler C.
        • Nemerovski M.
        Mol. Pharmacol. 1976; 12: 862-870