Advertisement

Insulin and dietary fructose induce stearoyl-CoA desaturase 1 gene expression of diabetic mice.

Open AccessPublished:November 04, 1994DOI:https://doi.org/10.1016/S0021-9258(18)47053-6
      This paper is only available as a PDF. To read, Please Download here.
      The transcription and mRNA levels of murine liver stearoyl-CoA desaturase 1 (SCD1) are induced 11- and 45-fold, respectively, by feeding fasted normal mice with a fat-free, high carbohydrate diet (Ntambi, J. M. (1992) J. Biol. Chem. 267, 10925-10930). In this study, we used streptozotocin-induced diabetic mice to study the regulatory role of carbohydrate and insulin on expression of the SCD1 gene in liver. Fructose administration to fasted diabetic mice induced a 2-fold increase in SCD1 mRNA within 6 h and a 23-fold increase within 24 h. Similarly, insulin administration to diabetic mice induced SCD1 mRNA from 4-fold within 4 h to 22-fold within 24 h. Insulin plus fructose, however, achieved full induction, with a 45-fold increase of SCD1 mRNA and a 10-fold increase in SCD1 transcription within 24 h. Additionally, the effect of insulin on SCD1 mRNA was inhibited 75% with dibutyryl-cAMP and theophylline administration and 70% by cycloheximide administration. Synthesis of liver albumin mRNA showed little change upon dietary manipulation or insulin treatment. Our data demonstrate that insulin and dietary fructose or a metabolite of fructose positively regulate the expression of the SCD1 gene in mouse liver.

      REFERENCES

        • Enoch H.G.
        • Catala A.
        • Strittmatter P.
        J. Biol. Chem. 1976; 251: 5095-5103
        • Thiede M.A.
        • Strittmatter P.
        J. Biol. Chem. 1985; 260: 14459-14463
        • Gellhorn A.
        • Benjamin W.
        Biochim. Biophys. Acta. 1964; 84: 167-175
        • Gellhorn A.
        • Benjamin W.
        Ann. N. Y. Acad. Sci. 1965; 131: 344-356
        • Prasad M.R.
        • Joshi V.C.
        J. Biol. Chem. 1979; 254: 997-999
        • DeTomas M.E.
        • Pelluffo R.O.
        • Mercuri O.
        Biochim. Biophys. Acta. 1973; 306: 149-155
        • Paulauskis J.D.
        • Sul H.S.
        J. Biol. Chem. 1989; 264: 574-577
        • Jump D.B.
        • Bell A.
        • Lepar G.
        • Hu D.
        Mol. Endocrinol. 1990; 4: 1655-1660
        • Noguchi T.
        • Inoue H.
        • Tanaka T.
        J. Biol. Chem. 1985; 260: 14393-14397
        • Decaux J.-E
        • Antoine B.
        • Kahn A.
        J. Biol. Chem. 1989; 264: 11584-11590
        • Katsurada A.
        • Iritani N.
        • Fukuda H.
        • Matsumura Y.
        • Noguchi T.
        • Tanaka T.
        Biochim. Biophys. Acta. 1989; 1004: 103-107
        • Thiede M.A.
        • Ozols J.
        • Strittmatter P.
        J. Biol. Chem. 1986; 261: 13230-13235
        • Ntambi J.M.
        J. Biol. Chem. 1992; 267: 10925-10930
        • Birnboim H.C.
        • Doly J.
        Nucleic Acids Res. 1979; 7: 1513-1523
        • Ziegler M.
        • Teneberg S.
        • Witt S.
        • Ziegler B.
        • Hehmke B.
        • Kohnert K.D.
        • Egeberg J.
        • Karlsson K.A.
        • Lemmark A.
        J. Immunol. 1988; 140: 4144-4150
        • Chirgwin J.M.
        • Przybyla A.E.
        • MacDonald R.J.
        • Rutter W.J.
        Biochemistry. 1979; 18: 5294-5299
        • Kaestner K.H.
        • Ntambi J.M.
        • Kelly Jr., T.J.
        • Lane M.D.
        J. Biol. Chem. 1989; 264: 14755-14761
        • Granner D.
        • Andreone T.
        • Sasaki K.
        • Beale E.
        Nature. 1983; 305: 549-551
        • Lamers W.H.
        • Hanson R.W.
        • Meisner H.M.
        Proc. Natl. Acad. Sci. U. S. A. 1982; 79: 5137-5141
        • Noguchi T.
        • Inoue H.
        • Tanaka T.
        Eur. J. Biochem. 1982; 128: 583-588
        • Munnich A.
        • Lyonnet S.
        • Chauvet D.
        • Van Schaftingen E.
        • Kahn A.
        J. Biol. Chem. 1987; 262: 17065-17071
        • Katsurada A.
        • Iritani N.
        • Fukuda H.
        • Noguchi T.
        • Tanaka T.
        Biochim. Biophys. Acta. 1988; 950: 113-117
        • Adelman R.C.
        • Spolter P.D.
        • Weinhouse S.
        J. Biol. Chem. 1966; 241: 5467-5472
        • Szepesi B.
        • Michaelis IV, O.E.
        Life Sci. 1972; 11: 113-119
        • Dioron B.
        • Cuif M.H.
        • Kahn A.
        • Diaz-Guerra M.J.M.
        J. Biol. Chem. 1994; 269: 10213-10216
        • Sasaki K.
        • Cripe T.P.
        • Koch S.R.
        • Andreone T.L.
        • Peterson D.D.
        • Beale E.G.
        • Granner D.K.
        J. Biol. Chem. 1984; 259: 15242-15251
        • Kasturi R.
        • Joshi V.C.
        J. Biol. Chem. 1982; 257: 12224-12230
        • Weiner F.R.
        • Smith P.J.
        • Wertheimer S.
        • Rubin C.S.
        J. Biol. Chem. 1991; 266: 23525-23528
        • Moustaid N.
        • Beyer R.S.
        • Sul H.S.
        J. Biol. Chem. 1994; 269: 5629-5634
        • Nasrin N.
        • Ercolani L.
        • Denaro M.
        • Kong X.F.
        • Kang I.
        • Alexander M.
        Proc. Natl. Acad. Sci. U. S. A. 1990; 87: 5273-5277