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Specific glucocorticoid receptors previously identified in rat thymus cells by measurements of steroid binding to whole cells are found to be located largely, if not entirely, in the nucleus. Localization is accomplished by showing that cortisol-3H, that is specifically bound to whole cells, remains with the nuclei (identified as such by electron microscopy) after cell membranes and cytoplasmic materials are removed by hypotonic shock in 1.5 mm MgCl2 at 3°. The cortisol-receptor complex has been extracted from nuclei and separated from free cortisol by gel chromatography. It is rapidly degraded by Pronase. The rate at which cortisol dissociated at 37° from the isolated complex, and from nuclei, is indistinguishable from the characteristic slow rate at which specifically bound cortisol dissociates from whole cells, showing that this latter rate is an intrinsic property of the cortisol-receptor complex. The rate at which cortisol associates specifically with whole cells at 37° is indistinguishable from the rate at which it appears in the nuclear receptor complex.
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Published online: July 10, 1970
Received:
April 30,
1970
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© 1970 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.
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