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Structure of the Phosphosaccharide-inositol Core of the Leishmania donovani Lipophosphoglycan

Open AccessPublished:April 25, 1989DOI:https://doi.org/10.1016/S0021-9258(18)83487-1
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      The phosphosaccharide-inositol core of the lipophosphoglycan of Leishmania donovani was generated by treatment of the glycoconjugate with mild acid and digestion with phosphatidylinositol-specific phospholipase C. The core was purified and examined by one- and two-dimensional 1H-1H NMR and by methylation analysis. From the results, the carbohydrate core was elucidated as a phosphosaccharide attached to the inositol residue of the lyso-alkylphosphatidylinositol anchor of lipophosphoglycan as follows: PO4→6GalP(α 1→6)GalP(α 1→3)Galf(α 1→3)ManP(α 1→3)ManP(α 1→4)GlcNP(α 1→6)myo-inositol. The presence of an internal galactofuranose residue is highly unusual and the ManP(α 1→4)GlcNP(α 1→6)myo-inositol sequence is homologous to the respective portion of the glycosylphosphatidylinositol anchors reported for both the Trypanosoma brucei variant surface glycoprotein and the rat brain Thy-1 glycoprotein.

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