Advertisement
Journal of Biological Chemistry
Open access logo
Advanced SearchSave search
Full length article| Volume 264, ISSUE 10, P5981-5987, April 05, 1989

Isolation and Partial Characterization of a Glial Hyaluronate-binding Protein

Open AccessPublished:April 05, 1989DOI:https://doi.org/10.1016/S0021-9258(18)83646-8
Isolation and Partial Characterization of a Glial Hyaluronate-binding Protein
Previous ArticleA Sulfated Glucosylceramide from Rat Kidney
Next ArticleRat Preprocarboxypeptidase H
      This paper is only available as a PDF. To read, Please Download here.
      A glial hyaluronate-binding protein (GHAP) with an isoelectric point of 4.3–4.4 was isolated from human brain white matter. The 60-kDa glycoprotein appeared to be quite resistant to proteolysis, and comparison with GHAP from a viable glioma removed at surgery showed that the protein isolated from autopsy material was not a degradation product resulting from postmortem autolysis. The protein was localized immunohistochemically with mouse monoclonal and rabbit polyclonal antibodies in cerebral white matter. Only small amounts could be found in the gray matter. After enzymatic deglycosylation, an immunoreactive 47-kDa polypeptide was obtained. Two amino acid sequences of GHAP showed a striking similarity (up to 89%) with a highly conserved region of cartilage proteins (bovine nasal cartilage proteoglycan and rat and chicken link protein). However, the amino acid composition and other amino acid sequences suggested that there are also differences between brain-specific GHAP and cartilage proteins.

      REFERENCES

        • Baker J.R.
        • Caterson B.
        J. Biol. Chem. 1979; 254: 2387-2393
        • Bignami A.
        • Dahl D.
        Proc. Natl. Acad. Sci. U. S. A. 1986; 83: 3518-3522
        • Bignami A.
        • Dahl D.
        J. Neurocytol. 1986; 15: 671-679
        • Bignami A.
        • Dahl D.
        Exp. Neurol. 1988; 99: 107-117
        • Bignami A.
        • Dahl D.
        J. Neuropathol. Exp. Neurol. 1988; 46 (abstr): 361
        • Bignami A.
        • Delpech B.
        Int. J. Dev. Neurosci. 1985; 3: 301-307
        • Bignami A.
        • Dahl D.
        • Gilad V.H.
        • Gilad G.M.
        Exp. Neurol. 1988; 100: 253-256
        • Caterson B.
        • Baker J.R.
        • Christner J.E.
        • Lee Y.
        • Lentz M.
        J. Biol. Chem. 1985; 260: 11348-11356
        • Caterson B.
        • Cababro T.
        • Donohue P.J.
        • Jahnke M.R.
        Kuettner K.E. Schleyerback R. Hascall V.C. Articular Cartilage Biochemistry. Raven Press, New York1986: 59-73
        • Celis J.E.
        • Bravo R.
        Two-dimensional Gel Electrophoresis of Proteins.
        Methods and Applications. Academic Press, Orlando, FL1985: 3-36
        • Dahl D.
        • Bignami A.
        Brain Res. 1973; 57: 343-360
        • Dahl D.
        • Bignami A.
        Cell Muscle Motil. 1985; 6: 75-96
        • Dayhoff M.O.
        • Barker W.C.
        • Hunt L.T.
        Methods Enzymol. 1983; 91: 524-525
        • Deák F.
        • Kiss I.
        • Sparks K.J.
        • Argraves W.S.
        • Hampikian G.
        • Goetinck P.F.
        Proc. Natl. Acad. Sci. U. S. A. 1986; 83: 3766-3770
        • Delpech A.
        • Delpech B.
        Dev. Biol. 1984; 101: 391-400
        • Delpech B.
        • Halavent C.
        J. Neurochem. 1981; 36: 855-859
        • Delpech B.
        • Vidard M.-N.
        • Delpech A.
        Immunochemistry. 1976; 13: 111-116
        • Delpech A.
        • Girard N.
        • Delpech B.
        Brain Res. 1982; 245: 251-257
        • Delpech B.
        • Bertrand P.
        • Hermelin B.
        • Delpech A.
        • Girard N.
        • Halkin E.
        • Chauzy C.
        Matrix Biol. 1986; 11: 78-89
        • Devereux J.
        • Haeberli P.
        • Smithies O.
        Nucleic Acids Res. 1984; 12: 387-395
        • Doege K.
        • Sasaki M.
        • Horigan E.
        • Hassell J.R.
        • Yamada Y.
        J. Biol. Chem. 1987; 262: 17757-17767
        • Ebert R.F.
        Anal. Biochem. 1986; 154: 431-435
        • Edge A.S.B.
        • Faltynek C.R.
        • Hof L.
        • Reichert Jr., L.E.
        • Weber P.
        Anal. Biochem. 1981; 118: 131-137
        • Glass W.F.
        Anal. Biochem. 1981; 115: 219-224
        • Goetinck P.F.
        • Stirpe N.S.
        • Tsonis P.A.
        • Carlone D.
        J. Cell Biol. 1987; 105: 2403-2408
        • Greenwood F.C.
        • Hunter W.M.
        • Glover J.S.
        Biochem. J. 1963; 89: 114-123
        • Hascall V.C.
        • Hascall G.K.
        Hay E.D. Cell Biology of the Extracellular Matrix. Plenum Publishing Corp., New York1981: 39-63
        • Laemmli U.K.
        Nature. 1970; 227: 680-685
        • Neame P.J.
        • Christner J.E.
        • Baker J.R.
        J. Biol. Chem. 1986; 261: 3519-3535
        • Neame P.J.
        • Christner J.E.
        • Baker J.R.
        J. Biol. Chem. 1987; 262: 17768-17778
        • Perin J.P.
        • Bennet F.
        • Zolles P.
        FEBS Lett. 1986; 205: 73-83
        • Tengblad A.
        Biochim. Biophys. Acta. 1979; 578: 281-289
        • Towbin H.
        • Staehelin T.
        • Gordon J.
        Proc. Natl. Acad. Sci. U. S. A. 1979; 76: 4350-4354

      Article Info

      Publication History

      Published online: April 05, 1989
      Received: April 14, 1988

      Identification

      DOI: https://doi.org/10.1016/S0021-9258(18)83646-8

      Copyright

      © 1989 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.

      User License

      Creative Commons Attribution (CC BY 4.0) |
      How you can reuse Information Icon
      Creative Commons Attribution (CC BY 4.0)

      Permitted

      • Read, print & download
      • Redistribute or republish the final article
      • Text & data mine
      • Translate the article
      • Reuse portions or extracts from the article in other works
      • Sell or re-use for commercial purposes

      Elsevier's open access license policy

      ScienceDirect

      Access this article on ScienceDirect

      ASBMB  ASBMB  ASBMB  ASBMB

      ISSN 0021-9258
      We use cookies to help provide and enhance our service and tailor content. To update your cookie settings, please visit the for this site.
      Copyright © 2022 Elsevier Inc. except certain content provided by third parties. The content on this site is intended for healthcare professionals.


      RELX