This paper is only available as a PDF. To read, Please Download here.
Activation of hGH-1 expression is mediated by the pituitary-specific transcription factor GHF-I/Pit-I which binds the 5‘-flanking DNA at two sites: I (-96/-70) and II (-134/-106). Although the factor(s) which direct the placental-specific expression of hCS-1 are not known, hCS-1 sequences are transcriptionally active in pituitary cells. In the present study we examined the effects of sequence differences between hGH-1 and hCS-1 5‘-flanking DNAs in determining their basal and thyroid hormone-regulated promoter activities. We showed that Sp1 is a major determinant of both hGH-1 and hCS-1 promoter activities and that in hGH-1, binding and activation by Sp1 are modulated by interference from GHF-I/Pit-1 binding at the adjacent site II sequence. A single base which differed in site II of hCS-1 greatly reduced GHF-1/Pit-1 binding and thus facilitated binding and activation by Sp1. Further differences in promoter activity of hGH-1 and hCS-1 sequences were accounted for by a thyroid hormone-responsive element between -62/-48 in the hCS-1 gene. However, induction by T3 was independent of either Sp1 or GH-1/Pit-1 binding in the site II region. These data demonstrate that a small number of base changes between hGH and hCS promoter sequences subserve a number of mechanisms which may differentially modulate the expression of hGH and hCS genes.
REFERENCES
- Nucleic Acids Res. 1983; 11: 3939-3958
- DNA (NY). 1987; 6: 59-70
- EMBO J. 1987; 6: 971-981
- Science. 1988; 239: 1400-1405
- Cell. 1987; 50: 267-275
- Cell. 1988; 55: 519-529
- Science. 1989; 243: 814-817
- Nature. 1989; 342: 945-948
- Cell. 1988; 55: 379-389
- Biochem. J. 1990; 267: 653-658
- UNA (NY). 1989; 8: 149-159
- Nucleic Acids Res. 1987; 15: 1297-1309
- Nucleic Acids Res. 1989; 17: 4237-4337
- Eur. J. Biochem. 1989; 181: 555-561
- Nucleic Acids Res. 1990; 18: 57-64
- J. Biol. Chem. 1987; 262: 6373-6382
- Nature. 1987; 329: 738-741
- Proc. Natl. Acad. Sei. U. S. A. 1987; 84: 5670-5674
- Mol. Endocrinol. 1988; 2: 792-798
- J. Biol. Chem. 1988; 263: 9418-9426
- J. Biol. Chem. 1989; 264: 12063-12073
- Sizonenko P.C. Aubert M.L. in Developmental Endocrinology. Raven Press, New York1990: 221-237
- J. Biol. Chem. 1986; 261: 13367-13372
- Endocrinol ogy. 1979; 105: 80-85
- Mol. Cell. Biol. 1982; 2: 1044-1051
- Nature. 1988; 332: 87-90
- Molecular Cloning: A Laboratory Manual. Second Ed, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York1989
- Proc. Natl. Acad. Sei. U. S. A. 1985; 82: 488-492
- Gene Anal. Tech. 1988; 5: 22-31
- Mol. Endocrinol. 1987; 1: 90-96
- J. Biol. Chem. 1988; 263: 9409-9417
- Genes & Dev. 1987; 1: 1147-1160
- Anal. Biochem. 1986; 156: 251-256
- Proc. Natl. Acad. Sci. U. S. A. 1983; 80: 5198-5202
- Mol. Endocrinol. 1989; 3: 1996-2004
- Mol. Cell. Biol. 1990; 10: 1811-1814
- Nature. 1989; 337: 659-661
- Nucleic Acids Res. 1988; 16: 1471-1485
- Annu. Rev. Physiol. 1990; 52: 773-791
- Trends Biochem. Sci. 1986; 11: 20-23
- Nucleic Acids Res. 1989; 7: 2639-2653
- J. Biol. Chem. 1988; 263: 3372-3379
- Nature 3 1. 1985; 7: 828-831
- EMBO J. 1988; 7: 3763-3770
- Nucleic Acids Res. 1987; 15: 4997-5016
- Proc. Natl. Acad. Sci. U. S. A. 1986; 83: 9021-9025
- Mol. Endocrinol. 1990; 4: 1293-1301
- Science. 1988; 242: 1418-1420
- J. Mol. Biol. 1986; 190: 379-389
- EMBOJ. 1988; 7: 3389-3395
- Mol. Endocrinol. 1989; 3: 1434-1442
- J. Biol Chem. 1990; 265: 2500-2504
- Mol. Cell. Biol. 1987; 7: 1193-1197
- Genomics. 1989; 4: 479-497
Article info
Publication history
Published online: May 25, 1991
Identification
Copyright
© 1991 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.
User license
Creative Commons Attribution (CC BY 4.0) | How you can reuse
Elsevier's open access license policy

Creative Commons Attribution (CC BY 4.0)
Permitted
- Read, print & download
- Redistribute or republish the final article
- Text & data mine
- Translate the article
- Reuse portions or extracts from the article in other works
- Sell or re-use for commercial purposes
Elsevier's open access license policy