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Diphtheria Toxin-dependent Adenosine Diphosphate Ribosylation of Aminoacyl Transferase II and Inhibition of Protein Synthesis

Open AccessPublished:June 25, 1968DOI:https://doi.org/10.1016/S0021-9258(18)93347-8
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      In the presence of diphtheria toxin, the adenosine diphosphate ribose portion of nicotinamide adenine dinucleotide was transferred to aminoacyl transferase II obtained from rat liver. The reaction resulted in a concurrent inactivation of this particular enzyme, one of the supernatant factors which assemble amino acids into polypeptide chain. The linkage of ADP-ribose to aminoacyl transferase II appeared to be of covalent nature. The reaction was reversible. Diptheria toxin may act as an enzyme or a catalytic cofactor in this reaction.

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