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ARTICLE| Volume 248, ISSUE 21, P7530-7539, November 10, 1973

Synthesis of a Deoxyribonucleic Acid Sequence Complementary to Ovalbumin Messenger Ribonucleic Acid and Quantification of Ovalbumin Genes

  • Drew Sullivan
    Affiliations
    From the Department of Biological Sciences, Stanford University, Stanford, California 94305, and the Department of Microbiology, University of California, Medical School, San Francisco, California 94122
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  • Rafael Palacios
    Affiliations
    From the Department of Biological Sciences, Stanford University, Stanford, California 94305, and the Department of Microbiology, University of California, Medical School, San Francisco, California 94122
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  • Janet Stavnezer
    Affiliations
    From the Department of Biological Sciences, Stanford University, Stanford, California 94305, and the Department of Microbiology, University of California, Medical School, San Francisco, California 94122
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  • John M. Taylor
    Affiliations
    From the Department of Biological Sciences, Stanford University, Stanford, California 94305, and the Department of Microbiology, University of California, Medical School, San Francisco, California 94122
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  • Anthony J. Faras
    Affiliations
    From the Department of Biological Sciences, Stanford University, Stanford, California 94305, and the Department of Microbiology, University of California, Medical School, San Francisco, California 94122
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  • Mary L. Kiely
    Affiliations
    From the Department of Biological Sciences, Stanford University, Stanford, California 94305, and the Department of Microbiology, University of California, Medical School, San Francisco, California 94122
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  • N. Morris Summers
    Affiliations
    From the Department of Biological Sciences, Stanford University, Stanford, California 94305, and the Department of Microbiology, University of California, Medical School, San Francisco, California 94122
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  • J. Michael Bishop
    Affiliations
    From the Department of Biological Sciences, Stanford University, Stanford, California 94305, and the Department of Microbiology, University of California, Medical School, San Francisco, California 94122
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  • Robert T. Schimke
    Affiliations
    From the Department of Biological Sciences, Stanford University, Stanford, California 94305, and the Department of Microbiology, University of California, Medical School, San Francisco, California 94122
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Open AccessPublished:November 10, 1973DOI:https://doi.org/10.1016/S0021-9258(19)43323-1
Synthesis of a Deoxyribonucleic Acid Sequence Complementary to Ovalbumin Messenger Ribonucleic Acid and Quantification of Ovalbumin Genes
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      DNA was synthesized in vitro from a template of purified ovalbumin messenger RNA. The DNA product is complementary to a homogeneous sequence specific for ovalbumin. The amount of ovalbumin sequence in hen oviduct and hen liver DNA was measured by hybridization experiments and found to be 2 copies per diploid genome in both tissues, indicating that there is no selective amplification of ovalbumin genes in the tissue specialized for obalbumin synthesis.

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      Article Info

      Publication History

      Published online: November 10, 1973
      Received: December 26, 1972

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      DOI: https://doi.org/10.1016/S0021-9258(19)43323-1

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      © 1973 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.

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