Advertisement

The Isolation of a New Hypotensive Peptide, Neurotensin, from Bovine Hypothalami

Open AccessPublished:October 10, 1973DOI:https://doi.org/10.1016/S0021-9258(19)43429-7
      This paper is only available as a PDF. To read, Please Download here.
      A hypotensive peptide, designated neurotensin, has been discovered and isolated in pure form from acid-acetone extracts of bovine hypothalami by column chromatography and paper electrophoresis. The results of amino acid analyses of material recovered after paper electrophoresis at pH 3.5, 6.5, and 8.9 and chromatographic analyses of the dansylated material indicate that the peptide isolated by this procedure is homogenous. Its amino acid composition and apparent molecular weight estimated by chromatography on Sephadex G-25 indicate that neurotensin is a tridecapeptide composed of Lys, Arg2, Asx, Glx2, Pro2, Ileu, Leu2, Tyr2. Neurotensin lacks a free NH2-terminus; however, it possesses a free COOH terminus which can be acted upon by carboxypeptidase A. Neurotensin induces hypotension in the rat and can stimulate the contraction of guinea pig ileum and rat uterus; however, it produces relaxation of the rat duodenum. These pharmacological properties classify it as a “kinin,” yet its chemical composition distinguishes it from any known peptide.

      REFERENCES

        • Leeman S.E.
        • Hammerschlag R.
        Endocrinology. 1967; 81: 803
        • Daynoff M.O.
        Allas of Protein Sequence and Structure. 5. National Biochemical Research Foundation, Maryland1972: D-173
        • Smith E.L.
        • Kimmel J.R.
        Boyer P.D. Lardy H. Mtrback K. The Enzymes. 4. Academic Press, New York1960: 133
        • Bailey J.L.
        Techniques in Protein Chemistry. Elsevier Publishing Co., New York1967: 101
        • Michl H.
        Monatsh. Chem. 1951; 82: 489
        • Bailey J.L.
        Techniques in Protein Chemistry. Elsevier Publishing Co., New York1967: 51
        • Reindel F.
        • Hoppe W.
        Chem. Ber. 1954; 87: 1103
        • Offord R.E.
        Nature. 1966; 211: 591
        • Mikes O.
        • Chalmers R.A.
        Laboratory Handbook of Chromatographic Methods. D. Van Nostrand Co., 1961: 63
        • Spaceman D.H.
        • Stein W.H.
        • Moore S.
        Anal. Chem. 1958; 30: 1190
        • Orten A.U.
        • Doppke H.J.
        • Spurrier H.H.
        Anal. Chem. 1965; 37: 623
        • Sanger F.
        • Thompson E.O.P.
        Biochim. Biophys. Acta. 1963; 71: 468
        • Needleman S.B.
        Protein Sequence Determination. Springer-Verlag, New York1970: 94
        • Hibs C.H.W.
        J. Biol. Chem. 1956; 219: 611
        • Gray W.R.
        Methods Enzymol. 1967; 11: 469
        • Gros C.
        • Laboucsse B.
        Eur. J. Biochem. 1969; 7: 463
        • Hartley B.S.
        Biochem. J. 1970; 119: 805
        • Udaka K.
        • Takeuchi Y.
        • Movat H.Z.
        Proc. Soc. Exp. Biol. Med. 1970; 133: 1384
        • Long J.A.
        • Evans H.M.
        The Oestrous Cycle in the Rat and Its Associated Phenomena. University of California Press, California1922
        • Berde B.
        Hanbuch de experimentellen pharmakologie. Springer-Verlag, Berlin1968: 155
        • Pernow B.
        Acta Physiol. Scand. 1953; 29
        • Chang M.M.
        • Leeman S.E.
        J. Biol. Chem. 1970; 245: 4784
        • Makino T.
        Abstracts. The Endocrine Society, 1973: 202
        • Carraway R.
        • Leeman S.E.
        Fed. Proc. 1971; 30: 117
        • Schally A.V.
        • Arimura A.
        • Kastin A.J.
        Science. 1973; 179: 341
        • Webster M.E.
        Erdos E.G. Back N. Sicateri F. Hypotensive Peptides. Springer-Verlag, New York1966: 648
        • Geller R.G.
        • Govier W.C.
        • Pisano J.J.
        • Tanimura T.
        • Van Clineschmidt B.
        Brit. J. Pharmacol. 1970; 40: 605
        • Lembeck F.
        Arch. Exp. Path. Pharmak. 1953; 219: 197
        • Otsuka M.
        • Konishi S.
        • Takahashi T.
        Proc. Jap. Acad. 1972; 48: 747
        • Weindl A.
        Abstracts. The Endocrine Society, 1971: 448
        • Chapman L.F.
        • Ramos A.O.
        • Goodell H.
        • Silverman G.
        • Wolff H.
        Arch. Neurol. 1960; 3: 223
        • Movat H.Z.
        Inflammation, Immunity, and Hyper sensitivity. Harper & Row, New York1971