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A hypotensive peptide, designated neurotensin, has been discovered and isolated in pure form from acid-acetone extracts of bovine hypothalami by column chromatography and paper electrophoresis. The results of amino acid analyses of material recovered after paper electrophoresis at pH 3.5, 6.5, and 8.9 and chromatographic analyses of the dansylated material indicate that the peptide isolated by this procedure is homogenous. Its amino acid composition and apparent molecular weight estimated by chromatography on Sephadex G-25 indicate that neurotensin is a tridecapeptide composed of Lys, Arg2, Asx, Glx2, Pro2, Ileu, Leu2, Tyr2. Neurotensin lacks a free NH2-terminus; however, it possesses a free COOH terminus which can be acted upon by carboxypeptidase A. Neurotensin induces hypotension in the rat and can stimulate the contraction of guinea pig ileum and rat uterus; however, it produces relaxation of the rat duodenum. These pharmacological properties classify it as a “kinin,” yet its chemical composition distinguishes it from any known peptide.
REFERENCES
- Endocrinology. 1967; 81: 803
- Allas of Protein Sequence and Structure. 5. National Biochemical Research Foundation, Maryland1972: D-173
- Boyer P.D. Lardy H. Mtrback K. The Enzymes. 4. Academic Press, New York1960: 133
- Elsevier Publishing Co., New York1967: 101 Techniques in Protein Chemistry.
- Monatsh. Chem. 1951; 82: 489
- Elsevier Publishing Co., New York1967: 51 Techniques in Protein Chemistry.
- Chem. Ber. 1954; 87: 1103
- Nature. 1966; 211: 591
- D. Van Nostrand Co., 1961: 63 Laboratory Handbook of Chromatographic Methods.
- Anal. Chem. 1958; 30: 1190
- Anal. Chem. 1965; 37: 623
- Biochim. Biophys. Acta. 1963; 71: 468
- Springer-Verlag, New York1970: 94 Protein Sequence Determination.
- J. Biol. Chem. 1956; 219: 611
- Methods Enzymol. 1967; 11: 469
- Eur. J. Biochem. 1969; 7: 463
- Biochem. J. 1970; 119: 805
- Proc. Soc. Exp. Biol. Med. 1970; 133: 1384
- The Oestrous Cycle in the Rat and Its Associated Phenomena. University of California Press, California1922
- Springer-Verlag, Berlin1968: 155 Hanbuch de experimentellen pharmakologie.
- Acta Physiol. Scand. 1953; 29
- J. Biol. Chem. 1970; 245: 4784
- The Endocrine Society, 1973: 202 Abstracts.
- Fed. Proc. 1971; 30: 117
- Science. 1973; 179: 341
- Erdos E.G. Back N. Sicateri F. Hypotensive Peptides. Springer-Verlag, New York1966: 648
- Brit. J. Pharmacol. 1970; 40: 605
- Arch. Exp. Path. Pharmak. 1953; 219: 197
- Proc. Jap. Acad. 1972; 48: 747
- The Endocrine Society, 1971: 448 Abstracts.
- Arch. Neurol. 1960; 3: 223
- Inflammation, Immunity, and Hyper sensitivity. Harper & Row, New York1971
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Published online: October 10, 1973
Received:
March 29,
1973
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© 1973 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.
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