This paper is only available as a PDF. To read, Please Download here.
Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. To investigate the role of CRABP in human skin we have cloned the human CRABP-II cDNA as well as the coding region of human CRABP-I. The predicted amino acid sequences of human CRABP-I and CRABP-II demonstrated a 99.3 and 93.5% identity to mouse CRABP-I and CRABP-II, respectively. CRABP-I transcripts were undetectable in adult human epidermis by RNA blot hybridization, while the CRABP-II cDNA probe detected an approximately 1.2-kilobase mRNA transcript. External application of 0.1% retinoic acid cream in vivo for 16 h resulted in a 16-fold induction of CRABP-II transcripts, while CRABP-I mRNA remained undetectable. Expression of CRABP-II, but not CRABP-I mRNA, was also markedly increased (greater than 15-fold) by retinoic acid treatment of fibroblasts cultured from human skin, whereas no significant induction of CRABP-II mRNA was observed in human lung fibroblasts. Human CRABP-II but not CRABP-I mRNA was significantly increased by agents which are known to induce keratinocyte differentiation in vitro. The marked inducibility of the CRABP-II gene is compatible with the idea that this isoform is important in retinoic acid-mediated regulation of human skin growth and differentiation.
References
- Cancer Res. 1983; 43: 3034-3040
- J. Cell Biol. 1989; 109: 295-307
- Dev. Biol. 1989; 133: 322-335
- Pharmacol. Ther. 1989; 40: 107-122
- Trends Genet. 1989; 5: 246-251
- Trends Neurosci. 1990; 13: 142-147
- J. Biol. Chem. 1985; 260: 6488-6493
- Proc. Natl. Acad. Sci. U. S. A. 1986; 83: 5779-5783
- Eur. J. Biochem. 1988; 173: 45-51
- Cancer Res. 1989; 49: 1497-1504
- Proc. Natl. Acad. Sci. U. S. A. 1990; 87: 6233-6237
- Nature. 1987; 330: 444-450
- Nature. 1988; 332: 850-853
- Nature. 1988; 333: 669-672
- Nature. 1989; 339: 714-717
- Proc. Natl. Acad. Sci. U. S. A. 1989; 86: 5310-5314
- Nature. 1990; 345: 224-229
- Arch. Biochem. Biophys. 1986; 247: 328-334
- Nature. 1988; 335: 733-735
- J. Invest. Dermatol. 1986; 86: 42-45
- Eur. J. Clin. Invest. 1989; 19: 220-227
- Arch. Dermatol. 1987; 123: 1690-1692
- Exp. Cell Res. 1988; 178: 114-126
- J. Cell. Physiol. 1990; 143: 294-302
- J. Invest. Dermatol. 1990; 94: 19-25
- Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY1982
- Proc. Natl. Acad. Sci. U. S. A. 1977; 74: 5463-5467
- Weber M.M. Sekely L.I. In Vitro Models for Cancer Research. 3. CRC Press, Boca Raton, FL1986: 245-274
- Science. 1979; 204: 526-527
- Nature. 1985; 314: 363-366
- Anal. Biochem. 1983; 132: 6-13
- DNA. 1988; 7: 261-267
- J. Biol. Chem. 1988; 263: 9326-9332
- Biochem. Biophys. Res. Commun. 1988; 157: 1302-1308
- Arch. Dermatol. 1972; 105: 695-701
- Mol. Cell. Biol. 1989; 9: 4846-4851
- J. Invest. Dermatol. 1991; 96: 425-433
Article info
Publication history
Published online: September 15, 1991
Identification
Copyright
© 1991 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.
User license
Creative Commons Attribution (CC BY 4.0) | How you can reuse
Elsevier's open access license policy

Creative Commons Attribution (CC BY 4.0)
Permitted
- Read, print & download
- Redistribute or republish the final article
- Text & data mine
- Translate the article
- Reuse portions or extracts from the article in other works
- Sell or re-use for commercial purposes
Elsevier's open access license policy