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Journal Article| Volume 256, ISSUE 8, P3978-3987, April 25, 1981

On the fidelity of DNA replication. Nucleoside monophosphate generation during polymerization.

Open AccessPublished:April 25, 1981DOI:https://doi.org/10.1016/S0021-9258(19)69555-4
On the fidelity of DNA replication. Nucleoside monophosphate generation during polymerization.
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      During catalysis by homogeneous procaryotic DNA polymerases, nucleoside monophosphates are generated by a 3' leads to 5'-exonucleolytic activity. Using Escherichia coli DNA polymerase I and poly[d(A-T)] as a template, the contribution of this activity to the fidelity of DNA synthesis has been evaluated by three different criteria. 1) The ratio between the rates of monophosphate generation and incorporation of the noncomplementary nucleotide with Mg2+ as an activating cation was 0.6 +/- 0.6, which is insufficient to account for the high fidelity of polymerization. 2) Inhibition of polymerization by pyrophosphate fails to diminish fidelity, although some kinetic models suggest that optimal error correction via monophosphate release requires the polymerization reaction to be strongly driven by pyrophosphate release. 3) The addition of deoxynucleoside monophosphates in concentrations as great as 10 mM to the reaction mixture does not alter the fidelity of DNA synthesis. These observations argue against the kinetic proofreading mode to account for the fidelity of E. coli DNA polymerase I when copying poly[d(A-T)] in a Mg2+-activated reaction. Furthermore, they suggest that the polymerase may enhance specificity at the base-selection step. However, the 3' leads to 5' exonuclease plays a larger role when the polymerase is activated with Mn2+ and may also be important in copying natural DNA where lower error rates are observed in vitro.

      REFERENCES

        • Watson J.D.
        • Crick F.H.C.
        Nature. 1953; 171: 737-738
        • Jordan F.
        • Sostman H.D.
        J. Am. Chem. Soc. 1973; 95: 6544-6554
        • Raszka M.
        • Kaplan N.O.
        Proc. Natl. Acad. Sci. U. S. A. 1972; 69: 2025-2029
        • Fresco J.R.
        • Alberts B.M.
        Proc. Natl. Acad. Sci. U.S. A. 1960; 46: 311-321
        • Wang A.C.
        • Kallenbach N.R.
        J. Mol. Biol. 1971; 62: 591-611
        • Clayton L.K.
        • Goodman M.F.
        • Branscomb E.W.
        • Galas D.J.
        J. Biol. Chem. 1979; 254: 1902-1912
        • Mildvan A.S.
        Annu. Rev. Biochem. 1974; 43: 357-399
        • Loeb L.A.
        • Springgate C.F.
        • Battula N.
        Cancer Res. 1974; 34: 2311-2321
        • Hopfield J.J.
        Proc. Natl. Acad. Sci. U. S. A. 1974; 71: 4135-4139
        • Agarwal S.S.
        • Dube D.K.
        • Loeb L.A.
        J. Biol. Chem. 1979; 254: 101-106
        • Trautner T.A.
        • Swartz M.N.
        • Kornberg A.
        Proc. Natl. Acad. Sci. U. S. A. 1962; 48: 449-455
        • Loftfield R.B.
        Biochem. J. 1963; 89: 82-92
        • Speyer J.F.
        Biochem. Biophys. Res. Commun. 1965; 21: 6-8
        • Drake J.W.
        • Allen E.F.
        Cold Spring Harbor Symp. Quant. Biol. 1968; 33: 339-344
        • Kornberg A.
        Science. 1969; 163: 1410-1418
        • Freese E.B.
        • Freese E.
        Proc. Natl. Acad. Sci. U. S. A. 1967; 57: 650-657
        • Koch A.L.
        • Miller C.
        J. Theor. Biol. 1965; 8: 71-80
        • Battula N.
        • Dube D.K.
        • Loeb L.A.
        J. Biol. Chem. 1975; 250: 8404-8408
        • Gillin F.D.
        • Nossal N.G.
        J. Biol. Chem. 1976; 251: 5225-5232
        • Sloan D.L.
        • Loeb L.A.
        • Mildvan A.S.
        • Feldmann R.J.
        J. Biol. Chem. 1975; 250: 8913-8920
        • Chang L.M.S.
        • Bollum F.J.
        J. Biol. Chem. 1973; 248: 3398-3404
        • Loeb L.A.
        Boyer P.D. The Enzymes. X. Academic Press, New York1974: 174-209
        • Battula N.
        • Loeb L.A.
        J. Biol. Chem. 1976; 251: 982-986
        • Springgate C.F.
        • Loeb L.A.
        J. Mol. Biol. 1975; 97: 577-591
        • Kornberg A.
        DNA Replication. W. H. Freeman and Co., San Francisco1980
        • Hall Z.W.
        • Lehman I.R.
        J. Mol. Biol. 1968; 36: 321-333
        • Brutlag D.
        • Kornberg A.
        J. Biol. Chem. 1972; 247: 241-248
        • Muzyczka N.
        • Poland R.L.
        • Bessman M.J.
        J. Biol. Chem. 1972; 247: 7116-7122
        • Hershfield M.S.
        J. Biol. Chem. 1973; 248: 1417-1423
        • Ninio J.
        Biochimie. 1975; 57: 587-595
        • Glickman B.
        • van den Elsen P.
        • Radman M.
        Mol. Gen. Genet. 1978; 163: 307-312
        • Moffatt G.
        Can. J. Chem. 1964; 42: 599-604
        • Battula N.
        • Loeb L.A.
        J. Biol. Chem. 1974; 249: 4086-4093
        • Radman M.
        • Villani G.
        • Boiteux S.
        • Kinsella A.R.
        • Glickman B.W.
        • Spadari S.
        Cold Spring Harbor Symp. Quant. Biol. 1979; 43: 937-946
        • Hershfield M.S.
        • Nossal N.G.
        J. Biol. Chem. 1972; 247: 3393-3404
        • Cohn M.
        • Townsend J.
        Nature. 1954; 173: 1090
        • Orgel A.
        • Orgel L.E.
        J. Mol. Biol. 1965; 14: 453-457
        • Sirover M.A.
        • Dube D.K.
        • Loeb L.A.
        J. Biol. Chem. 1979; 254: 107-111
        • Shapiro R.
        • Rlein R.S.
        Biochemistry. 1966; 5: 2358-2362
        • Runkel T.A.
        • Meyer R.R.
        • Loeb L.A.
        Proc. Natl. Acad. Sci. U. S. A. 1979; 26: 6331-6335
        • Deutscher M.P.
        • Kornberg A.
        J. Biol. Chem. 1969; 244: 3019-3028
        • Bernardi F.
        • Saghi M.
        • Dorizzi M.
        • Ninio J.
        J. Mol. Biol. 1979; 129: 93-112
        • Byrnes J.J.
        • Downey K.M.
        • Que B.G.
        • Lee Y.W.
        • Black V.L.
        • So A.G.
        Biochemistry. 1977; 16: 3740-3745
        • Travaglini E.C.
        • Mildvan A.S.
        • Loeb L.A.
        J. Biol. Chem. 1975; 250: 8647-8656
        • Wildenberg J.
        • Meselson M.
        Proc. Natl. Acad. Sci. U. S. A. 1975; 72: 2202-2206
        • Chang L.M.S.
        • Cassani G.R.
        • Bollum F.J.
        J. Biol. Chem. 1972; 247: 7718-7723
        • Gillin F.D.
        • Nossal N.G.
        J. Biol. Chem. 1976; 251: 5219-5224
        • Runkel T.A.
        • Loeb L.A.
        J. Biol. Chem. 1980; 255: 9961-9966

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      Published online: April 25, 1981

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      DOI: https://doi.org/10.1016/S0021-9258(19)69555-4

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      © 1981 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.

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