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Complete amino acid sequence of an immunomodulatory protein, ling zhi-8 (LZ-8)

An immunomodulator from a fungus, Ganoderma lucidium, having similarity to immunoglobulin variable regions*
  • S Tanaka
    Affiliations
    Biochemical Genetics Division, the Molecular Oncology Division, and Analytical Center, Meiji Institute of Health Science, Odawara, Kanagawa 250, Japan
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  • K Ko
    Affiliations
    Biochemical Genetics Division, the Molecular Oncology Division, and Analytical Center, Meiji Institute of Health Science, Odawara, Kanagawa 250, Japan
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  • K Kino
    Correspondence
    To whom correspondence should be addressed.
    Affiliations
    Biochemical Genetics Division, the Molecular Oncology Division, and Analytical Center, Meiji Institute of Health Science, Odawara, Kanagawa 250, Japan
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  • K Tsuchiya
    Affiliations
    Biochemical Genetics Division, the Molecular Oncology Division, and Analytical Center, Meiji Institute of Health Science, Odawara, Kanagawa 250, Japan
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  • A Yamashita
    Affiliations
    Biochemical Genetics Division, the Molecular Oncology Division, and Analytical Center, Meiji Institute of Health Science, Odawara, Kanagawa 250, Japan
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  • A Murasugi
    Affiliations
    Biochemical Genetics Division, the Molecular Oncology Division, and Analytical Center, Meiji Institute of Health Science, Odawara, Kanagawa 250, Japan
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  • S Sakuma
    Affiliations
    Biochemical Genetics Division, the Molecular Oncology Division, and Analytical Center, Meiji Institute of Health Science, Odawara, Kanagawa 250, Japan
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  • H Tsunoo
    Affiliations
    Biochemical Genetics Division, the Molecular Oncology Division, and Analytical Center, Meiji Institute of Health Science, Odawara, Kanagawa 250, Japan
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  • Author Footnotes
    * The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
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      The complete amino acid sequence of a novel immunomodulatory protein, ling zhi-8 (LZ-8), isolated from a fungus, Ganoderma lucidium (Kino, K., Yamashita, A., Yamaoka, K., Watanabe, J., Tanaka, S., Ko, K., Shimizu, K., and Tsunoo, H. (1989) J. Biol. Chem. 264, 472-478), was determined by protein sequencing. The polypeptide consists of 110 amino acid residues with an acetylated amino end and has a molecular mass of 12,420 Da including an amino-end blocking group. There is no attachment site for an Asn-linked oligosaccharide chain, consistent with the very low carbohydrate content of LZ-8. These results indicate that the native form of LZ-8 with a molecular mass of 24 kDa is a homodimer of the LZ-8 polypeptide whose sequence is described here. Furthermore, the LZ-8 chain shows considerable similarity to the variable region of immunoglobulin heavy chain both in its sequence and in its predicted secondary structure. The interesting possibility that LZ-8 is related to an ancestral protein of the immunoglobulin superfamily is also discussed.

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