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Heterogeneity of glutamine synthetase polypeptides in Neurospora crassa.

Open AccessPublished:March 25, 1980DOI:https://doi.org/10.1016/S0021-9258(19)85875-1
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      Purified preparations of Neurospora crassa glutamine synthetase contain two nonidentical polypeptides that can be separated by acrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and 7 M urea. These polypeptides are synthesized both in vivo and in a heterologous cell-free protein-synthesizing system. The data presented indicate that both polypeptides contain an active site for glutamine synthetase activity and suggest that there is not a precursor-product relationship between them.

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