Introduction

Results and discussion
Rif2 inhibits MRX-dependent activation of Tel1 kinase
The Xrs2 subunit of MRX is dispensable for Rif2's inhibitory activity in vitro

The N-terminal region of Rif2 is essential for its regulatory role

Rif2, but not Rif2-F8A, stimulates the ATPase activity of MRX

A new model for Rif2-mediated regulation of Tel1 kinase
Experimental procedures
DNA substrates
Protein expression and purification
Tel1 kinase assay
ATPase assay
GST-pulldown and ATP-binding experiments
Author contributions
Supplementary Material
References
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Article info
Publication history
Footnotes
This work was supported in part by the National Institutes of Health Grants GM118129 (to P. M. B.); GM098509 (to R. G.); and GM59413, P30-CA008748, and U54-OD020355 (to J. H. P.) and by an NSF Graduate Research Fellowship 2014157291 (to S. H.). J. H. P. is a consultant for Novus Biologicals, and a scientific advisor for Atropos Therapeutics. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
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