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Reply to Corbeil et al.: Deletion of the transmembrane protein Prom1b in zebrafish disrupts outer-segment morphogenesis and causes photoreceptor degeneration

  • Zhaojing Lu
    Affiliations
    Key Laboratory of Molecular Biophysics of Ministry of Education, Department of Genetics and Developmental Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China
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  • Xuebin Hu
    Affiliations
    Key Laboratory of Molecular Biophysics of Ministry of Education, Department of Genetics and Developmental Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China

    State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong 510060, China
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  • James Reilly
    Affiliations
    Department of Life Sciences, Glasgow Caledonian University, Glasgow G4 0BA, Scotland United Kingdom
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  • Danna Jia
    Affiliations
    Key Laboratory of Molecular Biophysics of Ministry of Education, Department of Genetics and Developmental Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China
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  • Fei Liu
    Affiliations
    Key Laboratory of Molecular Biophysics of Ministry of Education, Department of Genetics and Developmental Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China
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  • Shanshan Yu
    Affiliations
    Key Laboratory of Molecular Biophysics of Ministry of Education, Department of Genetics and Developmental Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China
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  • Xiliang Liu
    Affiliations
    Key Laboratory of Molecular Biophysics of Ministry of Education, Department of Genetics and Developmental Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China
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  • Shanglun Xie
    Affiliations
    Key Laboratory of Molecular Biophysics of Ministry of Education, Department of Genetics and Developmental Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China
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  • Zhen Qu
    Affiliations
    Key Laboratory of Molecular Biophysics of Ministry of Education, Department of Genetics and Developmental Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China
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  • Yayun Qin
    Affiliations
    Key Laboratory of Molecular Biophysics of Ministry of Education, Department of Genetics and Developmental Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China
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  • Yuwen Huang
    Affiliations
    Key Laboratory of Molecular Biophysics of Ministry of Education, Department of Genetics and Developmental Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China
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  • Yuexia Lv
    Affiliations
    Key Laboratory of Molecular Biophysics of Ministry of Education, Department of Genetics and Developmental Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China
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  • Jingzhen Li
    Affiliations
    Key Laboratory of Molecular Biophysics of Ministry of Education, Department of Genetics and Developmental Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China
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  • Pan Gao
    Affiliations
    Key Laboratory of Molecular Biophysics of Ministry of Education, Department of Genetics and Developmental Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China
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  • Fulton Wong
    Affiliations
    Department of Ophthalmology, Duke University School of Medicine, Durham, North Carolina 27710
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  • Xinhua Shu
    Affiliations
    Department of Life Sciences, Glasgow Caledonian University, Glasgow G4 0BA, Scotland United Kingdom
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  • Zhaohui Tang
    Affiliations
    Key Laboratory of Molecular Biophysics of Ministry of Education, Department of Genetics and Developmental Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China
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  • Mugen Liu
    Correspondence
    To whom correspondence should be addressed..
    Affiliations
    Key Laboratory of Molecular Biophysics of Ministry of Education, Department of Genetics and Developmental Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China
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Open AccessPublished:November 08, 2019DOI:https://doi.org/10.1074/jbc.RL119.011224
      We thank Corbeil et al. (
      • Corbeil D.
      • Fargeas C.A.
      • Jászai J.
      Deciphering the roles of prominins in the visual system.
      ) for their interest in our work.
      In our study, the gene ID of prom1a is 322857, and the reference sequence is NP_001108615.2. The gene ID of prom1b is 378834, and the reference sequence is NP_932337.1. The target sequence for prom1a knockout (see our paper (
      • Lu Z.
      • Hu X.
      • Reilly J.
      • Jia D.
      • Liu F.
      • Yu S.
      • Liu X.
      • Xie S.
      • Qu Z.
      • Qin Y.
      • Huang Y.
      • Lv Y.
      • Li J.
      • Gao P.
      • Wong F.
      • Shu X.
      • Tang Z.
      • Liu M.
      Deletion of the transmembrane protein Prom1b in zebrafish disrupts outer-segment morphogenesis and causes photoreceptor degeneration.
      ), Fig. 1) exists in all known splice variants.
      We too were intrigued by the lack of a phenotype in the prom1a knockout zebrafish. The simplest explanation is that the role of prom1a was compensated for by its homologue prom1b. However, we have no direct evidence to support this theory.
      We agree that several valid points have been raised, which deserve future investigation, such as the subcellular localization of prom1b in photoreceptors. However, we agree even more with the view that it is more important for our paper to show “the functional relevance of prom1b deficiency on the visual system.” That, in fact, is what we have shown in our paper (i.e. deletion of prom1b in zebrafish disrupts outer-segment morphogenesis and causes photoreceptor degeneration). In addition, deletion of prom1b prevents oligomerization and causes mislocalization of Prph2, which is an important protein for outer-segment morphogenesis.

      References

        • Corbeil D.
        • Fargeas C.A.
        • Jászai J.
        Deciphering the roles of prominins in the visual system.
        J. Biol. Chem. 2019; 294 (17166): 17166
        • Lu Z.
        • Hu X.
        • Reilly J.
        • Jia D.
        • Liu F.
        • Yu S.
        • Liu X.
        • Xie S.
        • Qu Z.
        • Qin Y.
        • Huang Y.
        • Lv Y.
        • Li J.
        • Gao P.
        • Wong F.
        • Shu X.
        • Tang Z.
        • Liu M.
        Deletion of the transmembrane protein Prom1b in zebrafish disrupts outer-segment morphogenesis and causes photoreceptor degeneration.
        J. Biol. Chem. 2019; 294 (31362982): 13953-13963