- Li S.
- Kanno S.
- Watanabe R.
- Ogiwara H.
- Kohno T.
- Watanabe G.
- Yasui A.
- Lieber M.R.
XRCC4-DNA ligase IV stimulates Artemis endonuclease activity on 3′ overhangs without DNA-PKcs
Artemis requires DNA-PKcs for cleavage of 5′ overhangs
XRCC4-DNA ligase IV alone does not stimulate Artemis hairpin nicking activity
A 3′-OH at a 3′ overhang is required for XRCC4-DNA ligase IV stimulation of Artemis
A 3′-OH but not a 5′-PO4 at both DNA ends is critical for XRCC4-DNA ligase IV ligation activity
The XRCC4-DNA ligase IV complex interferes with Artemis·DNA-PKcs action at 3′ overhangs
DNA end chemical configuration serves as a XRCC4-DNA ligase IV recognition element
A model for XRCC4-DNA ligase IV stimulation of Artemis endonuclease activity
- Malu S.
- De Ioannes P.
- Kozlov M.
- Greene M.
- Francis D.
- Hanna M.
- Pena J.
- Escalante C.R.
- Kurosawa A.
- Erdjument-Bromage H.
- Tempst P.
- Adachi N.
- Vezzoni P.
- Villa A.
- Aggarwal A.K.
- Cortes P.
Physiological relevance of DNA-PKcs-independent Artemis endonuclease activity in V(D)J recombination
Physiological relevance of DNA-PKcs-independent Artemis endonuclease activity in NHEJ
Oligonucleotides and DNA substrates
Protein expression and purification
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This work was supported, in whole or in part, by National Institutes of Health grants (to M. R. L.). The authors declare that they have no conflicts of interest with the contents of this article. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
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