If you don't remember your password, you can reset it by entering your email address and clicking the Reset Password button. You will then receive an email that contains a secure link for resetting your password
If the address matches a valid account an email will be sent to __email__ with instructions for resetting your password
Renal-Electrolyte Division, Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261Department of Cell Biology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, Pennsylvania 15261
Renal-Electrolyte Division, Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261Department of Cell Biology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261
* This work was supported, in whole or in part, by National Institutes of Health Grants R01 ES014701, R37 DK051391, R01 DK098204, R01 GM075061, T32 DK061296, P30 DK079307, and K01 DK90195. The authors declare that they have no conflicts of interest with the contents of this article. 1 Present address: Dept. of Pathology, Microbiology and Immunology, Vanderbilt University, Nashville, TN 37232.
The extracellular regions of epithelial Na+ channel subunits are highly ordered structures composed of domains formed by α helices and β strands. Deletion of the peripheral knuckle domain of the α subunit in the αβγ trimer results in channel activation, reflecting an increase in channel open probability due to a loss of the inhibitory effect of external Na+ (Na+ self-inhibition). In contrast, deletion of either the β or γ subunit knuckle domain within the αβγ trimer dramatically reduces epithelial Na+ channel function and surface expression, and impairs subunit maturation. We systematically mutated individual α subunit knuckle domain residues and assessed functional properties of these mutants. Cysteine substitutions at 14 of 28 residues significantly suppressed Na+ self-inhibition. The side chains of a cluster of these residues are non-polar and are predicted to be directed toward the palm domain, whereas a group of polar residues are predicted to orient their side chains toward the space between the knuckle and finger domains. Among the mutants causing the greatest suppression of Na+ self-inhibition were αP521C, αI529C, and αS534C. The introduction of Cys residues at homologous sites within either the β or γ subunit knuckle domain resulted in little or no change in Na+ self-inhibition. Our results suggest that multiple residues in the α subunit knuckle domain contribute to the mechanism of Na+ self-inhibition by interacting with palm and finger domain residues via two separate and chemically distinct motifs.
). Recent studies using heterologously expressed channels suggest that Na+ self-inhibition reflects a reduction in channel open probability and functions as an important mechanism that modulates ENaC gating (
), a mechanistic understanding of how external Na+ reduces channel activity is lacking.
Clues regarding allosteric changes that occur in association with extracellular Na+ binding and Na+ self-inhibition were provided by the resolved structure of acid-sensing ion channel 1 (ASIC1), a related ENaC/degenerin family member (
). The resolved homotrimeric ASIC1 structures reveal a highly organized extracellular region linked to transmembrane domains (Fig. 1). The extracellular region of each subunit is composed of distinct domains. The more peripheral thumb, finger, and knuckle domains are formed by α helices, and proximal domains (palm and β ball) are formed by β strands. The knuckle domain includes two short helices (α6 and α7) arranged in a helix-loop-helix conformation that is linked to the palm domain (
) suggested that the knuckle domain, together with the upper portions of the palm domain, forms a rigid structural scaffold, in contrast to the more flexible lower palm domain. A human ENaC polymorphism (αW493R), located at the junction of the palm and knuckle domains, activates human ENaC in association with diminished Na+ self-inhibition (
). These observations, taken together, are consistent with a role of this region in the allosteric regulation of ENaC.
We recently reported that in-frame deletion of exon 11 of the human α subunit, which encodes the helix-turn-helix component of the knuckle domain (Fig. 1C), dramatically increased whole cell ENaC currents and channel open probability, in association with a loss of the Na+ self-inhibition response (
). In this study, we investigated the functional roles of individual residues in the α subunit knuckle domain by performing cysteine-scanning mutagenesis and examining the effects of these substitutions on Na+ self-inhibition. We discovered that substitutions within a cluster of hydrophobic residues in the proximal half of the knuckle domain and within a stretch of polar residues in the distal half of this domain greatly dampen Na+ self-inhibition. Interestingly, the functional roles of α subunit knuckle domain residues are not shared by the homologous residues in the β or γ subunits, suggesting that specific sites in the α subunit knuckle domain modulate ENaC gating. Moreover, unlike the α subunit, β and γ subunits lacking the knuckle domain are not processed to mature forms, suggesting that the β and γ subunit knuckle domains are required for proper ENaC biogenesis.
Point mutations and deletions were introduced into mouse α, β, and γ ENaC cDNAs using the QuikChange II XL site-directed mutagenesis kit (Stratagene, La Jolla, CA). Target mutations were verified by DNA sequencing. Wild type (WT) and mutant ENaC cRNAs were synthesized by in vitro transcription using T3 RNA polymerase (Ambion, Inc.), purified by an RNA purification kit (Qiagen), and quantified by spectrophotometry.
ENaC Expression and Two-electrode Voltage Clamp
ENaC expression in Xenopus oocytes and current measurements by two-electrode voltage clamp were performed as reported previously (
). Stage V and VI oocytes with the follicle cell layer removed were injected with 0.5 ng of each subunit (α, β, and γ) cRNA in a volume of 50 nl and incubated at 18 °C in modified Barth's solution (MBS; 88 mm NaCl, 1 mm KCl, 2.4 mm NaHCO3, 15 mm HEPES, 0.3 mm Ca(NO3)2, 0.41 mm CaCl2, 0.82 mm MgSO4, 10 μg/ml streptomycin sulfate, 100 μg/ml gentamycin sulfate, pH 7.4). All experiments were performed at room temperature (20–24 °C) 20–52 h following injection. Oocytes were placed in a recording chamber from Warner Instruments (Hamden, CT) and perfused with a constant flow rate of 12–15 ml/min. The perfusion solution contained 110 mm NaCl, 2 mm KCl, 2 mm CaCl2, and 10 mm HEPES and had the pH adjusted at 7.4. Voltage clamp was performed using an Axoclamp 900A computer-controlled microelectrode amplifier and DigiData 1440A interface controlled by pClamp version 9.2 (Molecular Devices Corp., Sunnyvale, CA). The protocol for harvesting oocytes from Xenopus laevis was approved by the University of Pittsburgh institutional animal care and use committee.
Surface expression of αβγ ENaC in oocytes was determined using a mouse β or γ subunit with an extracellular FLAG epitope tag, as reported previously (
). Briefly, oocytes were injected with 2 ng/subunit of the three ENaC cRNAs with either the β or γ subunit containing an extracellular FLAG epitope tag (DYKDDDDK). The FLAG tag was placed immediately following residue Thr137 in the β subunit, as described previously (
). The γ subunit tag was generated by replacing residues Val131–Thr138 with a FLAG sequence. Robust amiloride-sensitive Na+ currents confirmed that ENaCs containing a FLAG-tagged β or γ subunit were functional. Two days after cRNA injection, a surface expression assay was performed on ice, except for the last step, which was at room temperature (20–24 °C). Following a 30-min incubation in antibiotic-free MBS supplemented with 1% bovine serum albumin (MBS/BSA), oocytes were incubated for 1 h with MBS/BSA supplemented with 1 μg/ml of a mouse monoclonal anti-FLAG antibody (M2, Sigma). Oocytes were then washed in MBS/BSA and incubated with MBS/BSA supplemented with 1 μg/ml of a horseradish peroxidase coupled to a secondary antibody (peroxidase-conjugated AffiniPure F(ab′)2 fragment goat anti-mouse IgG, Jackson ImmunoResearch, West Grove, PA) for 1 h. Cells were washed and transferred to MBS without BSA. Individual oocytes were placed in a 96-well plate, and 100 μl of SuperSignal ELISA Femto maximum sensitivity substrates (Thermo Scientific, Rockford, IL) was added to each well. Cells were then incubated at room temperature for 1 min, and chemiluminescence in relative light units was quantified in a GloMax-Multi+ detection system (Promega, Madison, WI).
Immunoprecipitation of ENaC Expressed in Xenopus Oocytes
Oocytes were injected with mouse ENaC cRNAs where either the β or γ subunit had both an N-terminal HA and C-terminal V5 tag. Epitope-tagged subunits were either WT or a knuckle domain deletion mutant, as indicated. 24 h after injection, 20 oocytes for each condition were transferred to a 1.5-ml conical tube with a snap cap, and all liquid was removed. Detergent solution (400 μl of 100 mm NaCl, 40 mm KCl, 1 mm EDTA, 10% glycerol, 1% Nonidet P-40, 0.4% deoxycholate, 20 mm HEPES, pH 7.4, with Calbiochem Protease Inhibitor Mixture III (EDTA-free)) was added to each tube before homogenization by pipetting up and down with a Pipetteman (Gilson) and then with a BD insulin syringe. The homogenate was mixed on a vortex for 30 s and centrifuged for 10 min (200 × g) in an Eppendorf 5417C centrifuge at 4 °C. The supernatant was centrifuged a second time for 10 min (200 × g) at 4 °C, and that resulting supernatant was centrifuged again for 10 min (20,800 × g) at 4 °C (20-μl aliquots were retained as “total extract”). The final supernatant was mixed with 400 μl of detergent solution and incubated overnight with end-over-end mixing with 40 μl of a 50% slurry of anti-V5 antibodies conjugated to beads (Bethyl). The beads were (i) washed twice with HEPES-buffered saline (10 mm HEPES, pH 7.4, 150 mm NaCl) containing 1% Triton X-100, (ii) washed once with HEPES-buffered saline containing 0.01% SDS, and (iii) washed once with HEPES-buffered saline. The washed beads were heated for 5 min at 95 °C with 30 μl of Bio-Rad 2× Laemmli sample buffer containing 0.14 m β-mercaptoethanol and spun, and the supernatant was loaded onto an SDS gel. Immunoblots were probed with anti-V5 antibodies as described previously (
). Briefly, a low [Na+] bath solution (NaCl-1; 1 mm NaCl, 109 mmN-methyl-d-glucamine, 2 mm KCl, 2 mm CaCl2, 10 mm HEPES, pH 7.4) was rapidly replaced with a high [Na+] bath solution (NaCl-110; 110 mm NaCl, 2 mm KCl, 2 mm CaCl2, 10 mm HEPES, pH 7.4), whereas the oocytes expressing ENaCs were continuously clamped to a membrane potential of −60 or −100 mV. Bath solution exchange was done with a Teflon valve computer-controlled perfusion system (AutoMate Scientific Inc., Berkeley, CA). At the end of each experiment, 10 μm amiloride was added to the bath solution to determine the amiloride-insensitive portion of the whole-cell current. The magnitude of Na+ self-inhibition was represented by the ratio of the steady-state current (Iss) to peak current (Ipeak). Iss was determined as the current at 40 s following the Ipeak. Both Iss and Ipeak were amiloride-sensitive currents. The time constants (τ) for Na+ self-inhibition were estimated by fitting the first 40-s current decay from Ipeak with a standard exponential function in the Clampfit software. To minimize complications due to variability in the Na+ self-inhibition response between batches of oocytes, the responses of WT channels were always tested in an alternating manner with mutants in the same batch of oocytes.
ENaC Degradation in Yeast
Yeast Saccharomyces cerevisiae strains were grown at 26 °C using standard methods, and medium preparation and transformation were performed as described (
). The same cloning strategy and restriction enzymes were used to construct pRS426GPD-αENaCΔknuckle-HA, pRS426GPD-βENaCΔknuckle-HA, and pRS426GPD-γENaCΔknuckle-HA from the knuckle deletion constructs. Cycloheximide chase analyses of the ENaC subunits were performed as published (
). Proteins were resolved by SDS-PAGE before Western blot analysis. The ENaC subunits were detected using horseradish peroxidase (HRP)-conjugated anti-HA antibodies (clone 3F10; Roche Applied Science) at a dilution of 1:5000. Western blots were also probed with anti-glucose-6-phosphate dehydrogenase (Sigma) rabbit antisera, which served as a loading control. The glucose-6-phosphate dehydrogenase primary antibody was detected with a donkey HRP-conjugated anti-rabbit IgG secondary antibody (GE Healthcare). The Western blot signals were imaged using enhanced chemiluminescence (Pierce) and visualized on a Bio-Rad ChemiDoc XRS+ system. Quantitation was carried out using ImageJ (version 1.44; National Institutes of Health).
ENaC Degradation in FRT Cells
Fisher rat thyroid (FRT) cells were kindly provided by Peter Snyder (University of Iowa). FRT cells were cultured in DMEM/F-12 medium supplemented with 8% fetal bovine serum. Cells were plated in plastic wells (12-well size from Costar, Corning, NY) and the next day (18 h) transfected using Lipofectamine 2000 with plasmids encoding α, β, and γ mouse ENaC subunits as described in the figure legends. In each case one subunit had both N-terminal HA and C-terminal V5 tags, whereas the other two had no epitope tags. Knuckle domains were deleted where indicated in epitope-tagged subunits. Cycloheximide (1000× stock in DMSO) was added to cells in fresh medium at a final concentration of 100 μg/ml (Calbiochem EMD Millipore, Billerica, MA). At 0, 1, 2, 4, or 6 h following the addition of cycloheximide, the cells were washed with Dulbecco's phosphate-buffered saline with Mg2+ and Ca2+ (Corning Cellgro, Manassas, VA). Cells were solubilized at 4 °C in detergent solution (0.4% sodium deoxycholate, 1% Nonidet P-40, 63 mm EDTA, and 50 mm Tris-HCl, pH 8) with 1% protease inhibitor mixture III (EMD Millipore, Billerica, MA; pH 7.4). ENaC was immunoprecipitated with anti-V5 antibodies conjugated to beads, as described previously (
), before SDS-PAGE and immunoblotting with anti-V5 antibodies. An aliquot of the initial cell extract (5%) was retained for SDS-PAGE and immunoblotting for β-actin.
Data are presented as mean ± S.E. Significance comparisons between WT and mutant groups expressed in the same batches of oocytes were performed with Student's t test. Data obtained in Xenopus oocytes were analyzed using Microsoft Excel 2013 and Origin Pro version 8.5 (OriginLab Corp., Northampton, MA). Data obtained in yeast and FRT cells were analyzed by two-way analysis of variance with Tukey post hoc test using SigmaPlot version 12 (Systat Software Inc., San Jose, CA).
We recently reported that deletion of the human α subunit residues encoded by exon 11 (αGlu500–αLys517; Fig. 1C) increased ENaC activity, reflecting a loss of Na+ self-inhibition (
). These residues form the double helix bundle of the knuckle domain (Fig. 1). We asked whether mouse ENaCs with a similar α subunit knuckle domain deletion (residues Gln526–Val548; underlined in Fig. 1C) also exhibit increased ENaC activity and a loss of Na+ self-inhibition. Mouse ENaCs with an α subunit Gln526–Val548 deletion (denoted as αΔK (αΔKnuckle)) had significantly larger ENaC currents (Fig. 2A) with no significant change in cell surface expression when expressed in oocytes, as compared with WT (Fig. 2B). The αΔK mutant exhibited a significant blunting of the Na+ self-inhibition response following transition from a low (1 mm) to a high (110 mm) Na+ bath (Fig. 3). The absence of Na+ self-inhibition is reflected by a significant increase in the steady state/peak current ratio (Iss/Ipeak) and a reduction in the rate of current decay following the peak current.
), deletions of the β or γ subunit knuckle domain (βΔK, ΔGlu466–Ile490; γΔK, ΔGlu483–Leu507, underlined in Fig. 1C) exhibited markedly reduced amiloride-sensitive currents and surface expression (Fig. 2). To demonstrate that β and γ subunits with knuckle domain deletions were expressed in oocytes, we examined whole cell expression of ENaC subunits in oocytes injected with cRNAs for αβ*ΔKγ or αβγ*ΔK (where the asterisk represents a V5 tag at the C terminus). WT αβ*γ or αβγ* expression was also examined in the same batches of oocytes for comparison (Fig. 2C). β*ΔK expression was slightly lower than that of WT β*, but the difference was not statistically significant (mutant/WT, 0.79 ± 0.18, n = 3, p > 0.05). In contrast, γ*ΔK expression was significantly less than that of WT γ* (mutant/WT, 0.46 ± 0.10, n = 3, p < 0.05). However, the reduced whole cell expression does not account for reduction of channel activity or surface expression. These results suggest that a reduction in protein expression was not primarily responsible for reduced channel activity and surface expression as a result of a β or γ knuckle domain deletion. We also found that mature forms of the β and γ subunit were seen with WT ENaC. These mature forms are a slower migrating (110-kDa) β subunit, reflecting N-glycan maturation, as well as a faster migrating (75-kDa) γ subunit, reflecting N-glycan maturation and proteolytic processing (
). Only immature forms of the β or γ subunit were observed when the knuckle domain was deleted.
The reduced levels of activity and surface expression observed with channels bearing the βΔK or γΔK mutant could reflect aberrant subunit folding associated with enhanced endoplasmic reticulum-associated degradation. We have shown previously that individual ENaC subunits expressed in yeast undergo fairly rapid endoplasmic reticulum-associated degradation (
). Therefore, we performed cycloheximide chase experiments of individual ENaC subunits in yeast to determine whether deletion of the knuckle domain accelerated the rate of subunit degradation. The logic underlying the use of the yeast system is that quantitative data can be acquired in a rapid and facile manner, and gross alterations in the folding of an individual channel subunit are readily apparent. Positive results would then be followed-up by more laborious studies in higher cells. However, we found that subunits with a deleted knuckle domain (αΔK, βΔK, or γΔK) exhibited rates of degradation similar to their WT counterparts (Fig. 4).
We next examined whether knuckle domain deletions affect ENaC subunit degradation when all three subunits are co-expressed in FRT cells. We previously showed that processing of ENaC subunits in the biosynthetic pathway includes furin-dependent cleavage of the α and γ subunits as well as maturation of N-glycans (
). We performed cycloheximide chase experiments in FRT cells expressing all three ENaC subunits, comparing WT channels with channels where one subunit lacked a knuckle domain (Fig. 5). Both full-length (95-kDa) and cleaved (65-kDa) forms of the α subunit were readily detected in WT and αΔKβγ channels (knuckle domain deletion reduces the corresponding molecular mass of the full-length and cleaved forms by ∼3 kDa). In contrast, we found only the forms corresponding to an immature β subunit (96 kDa, but not the slower migrating mature 110-kDa β subunit) and immature γ subunit (93 kDa, but not the 75-kDa cleaved γ subunit) when channels lacked the β or γ subunit knuckle domain, respectively. Deletion of the α subunit knuckle domain did not significantly affect degradation of the corresponding full-length (95-kDa) or cleaved (65-kDa) forms of the α subunit, although a significant difference was observed at one time point (2 h) for the mature 65-kDa form (Fig. 5B). Similarly, deletion of the β or γ subunit knuckle domain did not alter the rate of degradation of the β or γ subunit, although a significant difference was observed at one time point (1 h) for the immature 93-kDa γ subunit (Fig. 5G).
We also examined whole cell, steady-state expression levels of ENaCs in FRT cells expressing WT channels or channels where one subunit lacked a knuckle domain. Whereas WT and αΔKβγ channels had similar levels of the α subunit (normalized to β-actin expression at t = 0), the αβΔKγ and αβγΔK channels had significantly reduced steady-state levels of the β and γ subunit, respectively, when compared with the WT channel (Fig. 5J; p < 0.05).
Because a loss of the α subunit knuckle domain (αΔK) led to channel activation and a loss of Na+ self-inhibition, we examined whether there were specific residues in this domain that contributed to the observed phenotype. We systematically mutated individual residues within the α subunit knuckle domain (Gln520–Gly547) to Cys and examined the functional effects of these mutations. All of the mutants were functional when expressed in oocytes (FIGURE 6, FIGURE 7). Half of the mutations (14 of 28) significantly reduced the Na+ self-inhibition response, as reflected by an increased Iss/Ipeak, compared with WT. Of these 14 mutants that had an increased Iss/Ipeak, 11 also showed greater time constants, indicating a slowed Na+ self-inhibition response. Two mutants (P521C and T540C) had greater time constants than WT, but the differences were not significant. One mutant (K544C) showed a time constant indistinguishable from that of WT, suggestive of a selective effect of the mutation on the magnitude of Na+ self-inhibition.
We have previously observed subunit specificity regarding the effects of mutations on Na+ self-inhibition (
). Consequently, we examined whether knuckle domain mutations at specific sites exhibited subunit specificity. We chose three sites where α subunit knuckle domain Cys substitutions significantly reduced Na+ self-inhibition (αPro521, αIle529, and αSer534; FIGURE 6, FIGURE 7). When we expressed channels with Cys mutations in the corresponding sites in the β or γ subunit, Na+ self-inhibition was either similar to WT (mutations at the sites corresponding to αIle529 and αSer534) or was moderately reduced (mutations at the sites corresponding to αPro521; Fig. 8). Combined with the data presented above, these results suggest that α knuckle domain residues have an important role in the Na+ self-inhibition response when compared with their corresponding β and γ subunit residues.
The goal of this study was to explore the functional role of the extracellular knuckle domain and to identify specific residues involved in Na+ self-inhibition. As with the human α subunit (
), deletion of the mouse α subunit knuckle domain led to channel activation. The lack of change in ENaC surface expression when the mouse α subunit knuckle domain is deleted, in association with the markedly impaired Na+ self-inhibition response, suggests that channel activation reflects an increase in its open probability. In fact, we found that human ENaCs lacking the α subunit knuckle domain had an open probability that was significantly greater than WT (
), which does not appear to be due to an enhanced rate of channel degradation as assessed by cycloheximide chase assays. We did not observe enhanced degradation of individual subunits lacking their knuckle domain when expressed in the yeast system (Fig. 4) or when all three subunits (αβγ with an individual subunit lacking a knuckle domain) were expressed in FRT cells. Interestingly, the β or γ knuckle domain deletion prevented subunit maturation in both oocytes and FRT cells. Moreover, levels of both mutant subunits were significantly lower than WT in FRT cells, reflecting reduced protein synthesis and/or increased degradation. Together, these results suggest that channels with β and γ subunits lacking knuckle domains do not efficiently transit the Golgi complex or trans-Golgi network, where processing events associated with subunit maturation occur. Our findings suggest that the mutant channels are retained in the endoplasmic reticulum and undergo endoplasmic reticulum-associated degradation.
It is not clear why channels with an α subunit knuckle domain deletion do not exhibit a reduction in channel expression or maturation. One possible explanation is that during biogenesis, subunit-specific interactions with molecular chaperones, as we previously reported (
), make this distinction. These differences are probably not due to a deletion of an N-glycosylation site because all three knuckle domains have a putative N-glycosylation site although at slightly different locations.
The knuckle domain is formed by two α helices connected by a short loop. The sites where Cys substitutions alter Na+ self-inhibition, mapped onto the resolved ASIC1 structure, are present in both helices as well as in a loop preceding the helices and the loop connecting the helices (Fig. 9). Two residues precede and three are within the proximal α helix (Trp520, Pro521, Ile529, Phe530, and Leu533). These are all non-polar amino acids, and the side chains of corresponding residues within the ASIC1 crystal structure are directed toward a hydrophobic pocket between the knuckle and palm domains (Fig. 9, green side chains). Trp520 is conserved in the three ENaC subunits (Fig. 1), and substitutions at this site in the human (Trp493) or rat (Trp520) α subunit similarly stimulate channel activity (
). We speculate that this hydrophobic contact plays a role in the mechanism of Na+ self-inhibition.
How might alterations at these knuckle domain sites impair the allosteric changes associated with Na+ self-inhibition? In ASIC1, the knuckle domain and upper palm β-strands have been suggested to function as a structurally conserved scaffold (
). We suggest that the hydrophobic contacts between the knuckle and palm domains may function as an anchor to convey conformational changes initiated by Na+ binding to the channel gate. Mutation-induced disruption of this anchor may prevent the synchronized movements of the lower palm domain and thus sever the conformational wave. In agreement with this notion, we found that Na+ self-inhibition was disrupted by Cys substitutions at specific sites that are expected to disrupt hydrophobic packing with the upper palm domain.
Within the intervening loop and distal α helix, Cys substitution at nine sites (Ser534, Leu535, Asn537, Asn538, Tyr539, Thr540, Asn543, Lys544, and Gly547) in the α subunit knuckle domain significantly reduced Na+ self-inhibition (FIGURE 6, FIGURE 7). The first three sites (Ser534, Leu535, and Asn537) lack corresponding residues in ASIC1 and cannot be mapped to the ASIC1 crystal structure. The next five are sites where Cys substitutions impair Na+ self-inhibition correspond to ASIC1 residues with polar side chains that project toward the finger domain within the same subunit (Fig. 9). There is a low degree of homology between the finger domains in ASIC1 and ENaC subunits, and the α subunit has an additional 73 residues in its finger domain that are absent in ASIC1 (
). Therefore, it is difficult to infer specific knuckle and finger domain interactions based on the resolved ASIC1 structure. However, numerous studies have highlighted the importance of the finger domain in modulating ENaC activity, particularly in response to proteases and extracellular Na+ (
In summary, we identified multiple residues within the α subunit knuckle domain where Cys substitutions alter Na+ self-inhibition. We propose that the α subunit knuckle domain facilitates allosteric structural conformational changes that occur in response to the binding of extracellular Na+. Our results suggest that functionally important residues are organized into two distinct motifs that interact with the palm and finger domains via hydrophobic and polar interactions, respectively.
S. S. and T. R. K. conceived and coordinated the study and wrote the paper. J. C., E. C. R., and K. L. W. performed and/or analyzed the experiments shown in Figs. 2, 3, 6, 7, and 8. M. E. Y., T. M. B., and J. L. B. designed, performed, and/or analyzed the experiments shown in Fig. 4. C. L. K. and R. P. H. designed, performed, and/or analyzed the experiments shown in Figs. 2C and 5. All authors reviewed the results and approved the final version of the manuscript.
Role of epithelial sodium channels and their regulators in hypertension.