Neurotoxins as Molecular Probes
Photoaffinity Labeling Sodium Channels
Purification of Sodium Channels
Functional Reconstitution of Sodium Channels
Purification and Reconstitution of Calcium Channels
Subunit Architecture of Calcium Channels
Auxiliary Subunits of Sodium and Calcium Channels
Sodium Channel Structure in Two and Three Dimensions
Cloning and Expression
- Goldin A.L.
- Snutch T.
- Lübbert H.
- Dowsett A.
- Marshall J.
- Auld V.
- Downey W.
- Fritz L.C.
- Lester H.A.
- Dunn R.
- Catterall W.A.
- Davidson N.
- Goldin A.L.
- Snutch T.
- Lübbert H.
- Dowsett A.
- Marshall J.
- Auld V.
- Downey W.
- Fritz L.C.
- Lester H.A.
- Dunn R.
- Catterall W.A.
- Davidson N.
Ion Conductance and Selectivity in Calcium Channels
Looking Back and Forward
- A quantitative description of membrane current and its application to conduction and excitation in nerve.J. Physiol. 1952; 117: 500-544
- Sodium channels and gating currents.Physiol. Rev. 1981; 61: 644-683
- Ionic Channels of Excitable Membranes.3rd Ed. Sinauer Associates Inc., Sunderland, MA2001
- Neurotoxins that act on voltage-sensitive sodium channels in excitable membranes.Annu. Rev. Pharmacol. Toxicol. 1980; 20: 15-43
- Partial characterization of a tetrodotoxin-binding component from nerve membrane.Proc. Natl. Acad. Sci. U.S.A. 1972; 69: 3634-3637
- The binding of labelled saxitoxin to the sodium channels in nerve membranes.J. Physiol. 1973; 235: 783-804
- A new method for labelling saxitoxin and its binding to non-myelinated fibres of the rabbit vagus, lobster walking leg, and garfish olfactory nerves.J Physiol. 1976; 261: 477-494
- Two types of scorpion toxin receptor sites, one related to the activation, the other to the activation of the action potential sodium channel.Toxicon. 1982; 20: 9-16
- Binding of sea anemone toxin to receptor sites associated with gating system of sodium channel in synaptic nerve endings in vitro.Proc. Natl. Acad. Sci. U.S.A. 1980; 77: 1646-1650
- Covalent labeling of protein components of the sodium channel with a photoactivable derivative of scorpion toxin.Proc. Natl. Acad. Sci. U.S.A. 1980; 77: 639-643
- Neurotoxin binding to receptor sites associated with voltage-sensitive sodium channels in intact, lysed, and detergent-solubilized brain membranes.J. Biol. Chem. 1979; 254: 11379-11387
- Purification of the saxitoxin receptor of the sodium channel from rat brain.Proc. Natl. Acad. Sci. U.S.A. 1981; 78: 4620-4624
- The saxitoxin receptor of the sodium channel from rat brain: evidence for two nonidentical β subunits.J. Biol. Chem. 1982; 257: 13888-13891
- The sodium channel from rat brain: purification and subunit composition.J. Biol. Chem. 1984; 259: 1667-1675
- Hydrophobic properties of the β1 and β2 subunits of the rat brain sodium channel.J. Biol. Chem. 1987; 262: 11369-11374
- Reconstitution of the voltage-sensitive sodium channel of rat brain from solubilized components.J. Biol. Chem. 1981; 256: 11457-11463
- The sodium channel from rat brain: reconstitution of neurotoxin-activated ion flux and scorpion toxin binding from purified components.J. Biol. Chem. 1984; 259: 1676-1688
- Reconstitution of neurotoxin-stimulated sodium transport by the voltage-sensitive sodium channel purified from rat brain.J. Biol. Chem. 1982; 257: 11868-11871
- Single Na+ channel currents observed in cultured rat muscle cells.Nature. 1980; 287: 447-449
- Functional reconstitution of the purified brain sodium channel in planar lipid bilayers.Proc. Natl. Acad. Sci. U.S.A. 1985; 82: 240-244
- Identification of a large molecular weight peptide associated with a tetrodotoxin binding proteins from the electroplax of Electrophorus electricus.Biochem. Biophys. Res. Commun. 1980; 92: 860-866
- Protein components of the purified sodium channel from rat skeletal muscle sarcolemma.J Neurochem. 1983; 40: 1377-1385
- Solubilization of the calcium antagonist receptor from rat brain.J. Biol. Chem. 1983; 258: 7280-7283
- Purification of the calcium antagonist receptor of the voltage-sensitive calcium channel from skeletal muscle transverse tubules.Biochemistry. 1984; 23: 2113-2118
- Reconstitution of the voltage-sensitive calcium channel purified from skeletal muscle transverse tubules.Biochemistry. 1986; 25: 3077-3083
- Purified dihydropyridine-binding site from skeletal muscle t-tubules is a functional calcium channel.Nature. 1986; 323: 66-68
- Subunit structure of dihydropyridine-sensitive calcium channels from skeletal muscle.Proc. Natl. Acad. Sci. U.S.A. 1987; 84: 5478-5482
- Structural characterization of the 1,4-dihydropyridine receptor of the voltage-dependent Ca2+ channel from rabbit skeletal muscle: evidence for two distinct high molecular weight subunits.J. Biol. Chem. 1987; 262: 7943-7946
- Photoaffinity labelling and phosphorylation of a 165 kilodalton peptide associated with dihydropyridine and phenylalkylamine-sensitive calcium channels.Biochem. Biophys. Res. Commun. 1987; 147: 1137-1145
- Reconstitution of the purified receptor for calcium channel blockers.Biomed. Biochim. Acta. 1987; 46: S357-S362
- The 165-kDa peptide of the purified skeletal muscle dihydropyridine receptor contains the known regulatory sites of the calcium channel.Eur. J. Biochem. 1987; 167: 117-122
- Photoaffinity labelling of the phenylalkylamine receptor of the skeletal muscle transverse-tubule calcium channel.FEBS Lett. 1987; 212: 247-253
- β2 subunits of sodium channels from vertebrate brain: studies with subunit-specific antibodies.J. Biol. Chem. 1987; 262: 14709-14715
- β1 subunits of sodium channels: studies with subunit-specific antibodies.J. Biol. Chem. 1990; 265: 12393-12399
- Biosynthesis and processing of the α subunit of the voltage-sensitive sodium channel in rat brain neurons.Cell. 1986; 46: 437-444
- Primary structure and functional expression of the β1 subunit of the rat brain sodium channel.Science. 1992; 256: 839-842
- Structure and function of the β2 subunit of brain sodium channels, a transmembrane glycoprotein with a CAM motif.Cell. 1995; 83: 433-442
- Auxiliary subunits of voltage-gated ion channels.Neuron. 1994; 12: 1183-1194
- The roles of the subunits in the function of the calcium channel.Science. 1991; 253: 1553-1557
- Na Channel β subunits: overachievers of the ion channel family.Front Pharmacol. 2011; 2: 53
- α2δ expression sets presynaptic calcium channel abundance and release probability.Nature. 2012; 486: 122-125
- Calcium channel auxiliary α2δ and β subunits: trafficking and one step beyond.Nat. Rev. Neurosci. 2012; 13: 542-555
- Primary structure of Electrophorus electricus sodium channel deduced from cDNA sequence.Nature. 1984; 312: 121-127
- Existence of distinct sodium channel messenger RNAs in rat brain.Nature. 1986; 320: 188-192
- Molecular cloning of a putative tetrodotoxin-resistant rat heart Na+ channel isoform.Proc. Natl. Acad. Sci. U.S.A. 1989; 86: 8170-8174
- Primary structure and functional expression of a mammalian skeletal muscle sodium channel.Neuron. 1989; 3: 33-49
- Genomic organization and deduced amino acid sequence of a putative sodium channel gene in Drosophila.Science. 1987; 237: 744-749
- Messenger RNA coding for only the α subunit of the rat brain Na channel is sufficient for expression of functional channels in Xenopus oocytes.Proc. Natl. Acad. Sci. U.S.A. 1986; 83: 7503-7507
- A rat brain sodium channel α subunit with novel gating properties.Neuron. 1988; 1: 449-461
- Expression of functional sodium channels from cloned cDNA.Nature. 1986; 322: 826-828
- From ionic currents to molecular mechanisms: the structure and function of voltage-gated sodium channels.Neuron. 2000; 26: 13-25
- A prokaryotic voltage-gated sodium channel.Science. 2001; 294: 2372-2375
- The crystal structure of a voltage-gated sodium channel.Nature. 2011; 475: 353-358
- Voltage clamp analysis of sodium channels in normal and scorpion toxin-resistant neuroblastoma cells.J. Neurosci. 1984; 4: 2836-2842
- Functional properties of rat brain sodium channels expressed in a somatic cell line.Science. 1990; 247: 854-858
- Identification of an intracellular peptide segment involved in sodium channel inactivation.Science. 1988; 241: 1658-1661
- Tissue-specific expression of the RI and RII sodium channel subtypes.Proc. Natl. Acad. Sci. U.S.A. 1987; 84: 8682-8686
- Biochemical properties of sodium channels in a wide range of excitable tissues studied with site-directed antibodies.Biochemistry. 1988; 27: 7032-7038
- Currents related to movement of the gating particles of the sodium channels.Nature. 1973; 242: 459-461
- Gating of the bacterial sodium channel, NaChBac: voltage-dependent charge movement and gating currents.J. Gen. Physiol. 2004; 124: 349-356
- Transfer of twelve charges is needed to open skeletal muscle Na+ channels.J. Gen. Physiol. 1995; 106: 1053-1068
- Voltage-dependent gating of sodium channels: correlating structure and function.Trends Neurosci. 1986; 9: 7-10
- Molecular properties of voltage-sensitive sodium channels.Annu. Rev. Biochem. 1986; 55: 953-985
- Molecular model of the action potential sodium channel.Proc. Natl. Acad. Sci. U.S.A. 1986; 83: 508-512
- Ion channel voltage sensors: structure, function, and pathophysiology.Neuron. 2010; 67: 915-928
- Structural parts involved in activation and inactivation of the sodium channel.Nature. 1989; 339: 597-603
- Binding of scorpion toxin to receptor sites associated with sodium channels in frog muscle: correlation of voltage-dependent binding with activation.J. Gen. Physiol. 1979; 74: 375-391
- Molecular determinants of high affinity binding of α-scorpion toxin and sea anemone toxin in the S3-S4 extracellular loop in domain IV of the Na+ channel α subunit.J. Biol. Chem. 1996; 271: 15950-15962
- Voltage sensor-trapping: enhanced activation of sodium channels by β-scorpion toxin bound to the S3-S4 loop in domain II.Neuron. 1998; 21: 919-931
- Evidence for voltage-dependent S4 movement in sodium channel.Neuron. 1995; 15: 213-218
- Molecular basis of charge movement in voltage-gated sodium channels.Neuron. 1996; 16: 113-122
- Disulfide locking a sodium channel voltage sensor reveals ion pair formation during activation.Proc. Natl. Acad. Sci. U.S.A. 2008; 105: 15142-15147
- Sequential formation of ion pairs during activation of a sodium channel voltage sensor.Proc. Natl. Acad. Sci. U.S.A. 2009; 106: 22498-22503
- Gating charge interactions with the S1 segment during activation of a Na+ channel voltage sensor.Proc. Natl. Acad. Sci. U.S.A. 2011; 108: 18825-18830
- Structural basis for gating charge movement in the voltage sensor of a sodium channel.Proc. Natl. Acad. Sci. U.S.A. 2012; 109: E93-E102
- Multipass membrane protein structure prediction using Rosetta.Proteins. 2006; 62: 1010-1025
- Voltage sensor conformations in the open and closed states in ROSETTA structural models of K+ channels.Proc. Natl. Acad. Sci. U.S.A. 2006; 103: 7292-7297
- Crystal structure of an orthologue of the NaChBac voltage-gated sodium channel.Nature. 2012; 486: 130-134
- Mapping of scorpion toxin receptor sites at voltage-gated sodium channels.Toxicon. 2012; 60: 502-511
- Structure-function map of the receptor site for β-scorpion toxins in domain II of voltage-gated sodium channels.J. Biol. Chem. 2011; 286: 33641-33651
- Mapping the interaction site for a β-scorpion toxin in the pore module of domain III of voltage-gated Na+ channels.J. Biol. Chem. 2012; 287: 30719-30728
- Mapping the receptor site for α-scorpion toxins on a Na+ channel voltage sensor.Proc. Natl. Acad. Sci. U.S.A. 2011; 108: 15426-15431
- Catalysis of Na+ permeation in the bacterial sodium channel NavAb.Proc. Natl. Acad. Sci. U.S.A. 2013; 110: 11331-11336
- Chemistry of ion coordination and hydration revealed by a potassium channel-Fab complex at 2.0 Å resolution.Nature. 2001; 414: 43-48
- Destruction of sodium conductance inactivation in squid axons perfused with Pronase.J. Gen. Physiol. 1973; 62: 375-391
- Inhibition of inactivation of single sodium channels by a site-directed antibody.Proc. Natl. Acad. Sci. U.S.A. 1989; 86: 8147-8151
- A cluster of hydrophobic amino acid residues required for fast Na+-channel inactivation.Proc. Natl. Acad. Sci. U.S.A. 1992; 89: 10910-10914
- Movement of the Na+ channel inactivation gate during inactivation.J. Biol. Chem. 1996; 271: 30971-30979
- Molecular analysis of the putative inactivation particle in the inactivation gate of brain type IIA Na+ channels.J. Gen. Physiol. 1997; 109: 589-605
- Molecular analysis of potential hinge residues in the inactivation gate of brain type IIA Na+ channels.J. Gen. Physiol. 1997; 109: 607-617
- Solution structure of the sodium channel inactivation gate.Biochemistry. 1999; 38: 855-861
- The effects of external potassium and long duration voltage conditioning on the amplitude of sodium currents in the giant axon of the squid, Loligo pealei.J. Gen. Physiol. 1969; 54: 589-606
- Slow inactivation of the sodium conductance in squid giant axons: Pronase resistance.J. Physiol. 1978; 283: 1-21
- The pore, not cytoplasmic domains, underlies inactivation in a prokaryotic sodium channel.Biophys. J. 2005; 89: 232-242
- Crystal structure of a voltage-gated sodium channel in two potentially inactivated states.Nature. 2012; 486: 135-139
- The VGL-chanome: a protein superfamily specialized for electrical signaling and ionic homeostasis.Sci. STKE. 2004; 2004: re15
- Structural basis for Ca2+ selectivity of a voltage-gated calcium channel.Nature. 2014; 505: 56-61
- Mechanism of ion permeation through calcium channels.Nature. 1984; 309: 453-456
- The nonselective conductance due to calcium channels in frog muscle: calcium-selectivity in a single file pore.J. Physiol. 1984; 353: 585-608
- A nonselective cation conductance in frog muscle membrane blocked by micromolar external calcium ions.J. Physiol. 1984; 353: 565-583
- Structural basis for pharmacology of voltage-gated sodium and calcium channels.Mol. Pharmacol. 2015; 88: 141-150
- Neuromodulation of Na+ channels: an unexpected form of cellular plasticity.Nat. Rev. Neurosci. 2001; 2: 397-407
- Structure and regulation of voltage-gated calcium channels.Annu. Rev. Cell Dev. Biol. 2000; 16: 521-555
- Signaling complexes of voltage-gated sodium and calcium channels.Neurosci. Lett. 2010; 486: 107-116
- Calcium channel regulation and presynaptic plasticity.Neuron. 2008; 59: 882-901
- The primary periodic paralyses: diagnosis, pathogenesis and treatment.Brain. 2006; 129: 8-17
- The channelopathies: novel insights into molecular and genetic mechanisms of human disease.J. Clin. Invest. 2005; 115: 1986-1989
- Channelopathies in idiopathic epilepsy.Neurotherapeutics. 2007; 4: 295-304
- Sodium channels in normal and pathological pain.Annu. Rev. Neurosci. 2010; 33: 325-347
- A Cacna1a knockin migraine mouse model with increased susceptibility to cortical spreading depression.Neuron. 2004; 41: 701-710
- Gating pore current in an inherited ion channelopathy.Nature. 2007; 446: 76-78
- Depolarization-activated gating pore current conducted by mutant sodium channels in potassium-sensitive normokalemic periodic paralysis.Proc. Natl. Acad. Sci. U.S.A. 2008; 105: 19980-19985
- Ion permeation and block of the gating pore in the voltage sensor of NaV1.4 channels with hypokalemic periodic paralysis mutations.J. Gen. Physiol. 2010; 136: 225-236
- Reduced sodium current in GABAergic interneurons in a mouse model of severe myoclonic epilepsy in infancy.Nat. Neurosci. 2006; 9: 1142-1149
- NaV1.1 channels and epilepsy.J. Physiol. 2010; 588: 1849-1859
- Autistic-like behaviour in Scn1a+/− mice and rescue by enhanced GABA-mediated neurotransmission.Nature. 2012; 489: 385-390
- Basal and β-adrenergic regulation of the cardiac calcium channel CaV1.2 requires phosphorylation of serine 1700.Proc. Natl. Acad. Sci. U.S.A. 2014; 111: 16598-16603
- Phosphorylation sites required for regulation of cardiac calcium channels in the fight-or-flight response.Proc. Natl. Acad. Sci. U.S.A. 2013; 110: 19621-19626
- The case of the CIA and butter clam toxin.Yale Medicine. Yale School of Medicine, New Haven, CT2000–2001 (Vol. 35, No. 1 Fall-Winter)
- Subunits of purified calcium channels: α2 and δ are encoded by the same gene.J. Biol. Chem. 1990; 265: 14738-14741
- The α2δ subunits of voltage-gated calcium channels form GPI-anchored proteins, a posttranslational modification essential for function.Proc. Natl. Acad. Sci. U.S.A. 2010; 107: 1654-1659
User LicenseCreative Commons Attribution (CC BY 4.0) |
- Read, print & download
- Redistribute or republish the final article
- Text & data mine
- Translate the article
- Reuse portions or extracts from the article in other works
- Sell or re-use for commercial purposes
Elsevier's open access license policy