September 04, 2015
Volume 290Issue 36p21773-22311
Open Access
On The Cover: Antigen-binding fragment (Fab) of an antibody depicted as a sphere model (cyan) is engineered to bind two structurally unrelated angiogenic factors, vascular endothelial
growth factor (orange) and angiopoietin 2 (pink). This dual action Fab achieved sub-nanomolar affinity for both antigens, a feat
enabled by deep mutational scanning, which measured the effect of all possible mutations
of each CDR residue depicted by heat map (left background) and identified synergistic mutation pairs by cooperativity analysis represented
with the Circos plot (right background). For details see the article by Koenig et al., pages 21773–21786. The cover submission was designed by Allison Bruce....Show more
On The Cover: Antigen-binding fragment (Fab) of an antibody depicted as a sphere model (cyan) is engineered to bind two structurally unrelated angiogenic factors, vascular endothelial
growth factor (orange) and angiopoietin 2 (pink). This dual action Fab achieved sub-nanomolar affinity for both antigens, a feat
enabled by deep mutational scanning, which measured the effect of all possible mutations
of each CDR residue depicted by heat map (left background) and identified synergistic mutation pairs by cooperativity analysis represented
with the Circos plot (right background). For details see the article by Koenig et al., pages 21773–21786. The cover submission was designed by Allison Bruce.
