In a recent article (1), the authors examine the binding of lab-evolved trans-activation response (TAR)–binding proteins (TBPs) to HIV-1 TAR RNA. Here, we show our analysis of the thermodynamic data of the binding that identifies three quantitative features of the binding, which may provide further insight into the interactions.where ΔH° and ΔS° are experimentally measured values reported in the original paper (1). Based on the statistical parameters, probability density of the ΔG° can be generated using Equation 2:where σ = 1.9407 kJ/mol and ΔG°mean = –41.3 kJ/mol.
